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For research use only.
SOLIScript® 1-step SolisGreen® Kit is an optimized solution for highlysensitive real-time one-step RT-PCR (RT-qPCR) using intercalatingdye detection via the SYBR®/FAM fluorescence channel.
SOLIScript® 1-step SolisGreen® Kit is optimized for highly sensitive and accurate quantification of RNA targets by real-time one-step RT-PCR (RT-qPCR) using dsDNA-intercalating dye detection via the SYBR®/FAM fluorescence channel.
Compatible with ROX-independent and ROX-dependent qPCR cyclers (excluding high-ROX platforms).
The Kit comes in 3 tubes (including water) and contains all the components necessary (except RNA template and primers) to perform cDNA synthesis and qPCR in a single tube.
Sample type: RNA
Product format: Reagent kit
No of tubes: three tubes
Detection type: Intercalating dye-based
Reference dye: reference dye based on ROX
Compatible qPCR cyclers: The Kit is compatible with ROX-independent and ROX-dependent qPCR platforms (excluding high-ROX machines, such as Applied Biosystems™ 5700, 7000, 7700, 7900HT, StepOne™, StepOnePlus™). Check Your cycler!
40x One-step SOLIScript® Mix: SOLIScript® Reverse Transcriptase, RiboGrip™ RNase Inhibitor
5x One-step SolisGreen® Mix: HOT FIREPol® DNA polymerase, dNTPs (dATP, dCTP, dGTP, dTTP), 12.5 mM MgCl2 (1x RT-qPCR solution – 2.5 mM MgCl2), SolisGreen® dsDNA-intercalating dye, passive reference dye based on ROX dye
RiboGrip™ is not directly associated with tolerance to common inhibitors, however, it helps to keep RNA intact and prevent RNA degradation because RiboGrip is inactivating ribonuclease enzymes
SolisGreen™ nucleic acid dye shares spectral properties with other commonly used qPCR dyes, allowing detection in the FAM or SYBR® Green I channel (SolisGreen™ excitation maximum 489 nm, emission maximum 515 nm). SolisGreen™ is characterized by high sensitivity and great PCR efficiency with low template amounts for accurate and reproducible results.
SolisGreen™ is based on CYGREEN dye. CYGREEN is used under licence from Enzo Life Sciences, Inc. CYGREEN is a U.S. registered trademark of Enzo Life Sciences, Inc. U.S. Patent Nos. 8,153,802 and 7,569,695.
Both dyes are DNA intercalating agents that are used to stain dsDNA. SolisGreen™ dye is spectrally similar to EvaGreen® allowing detection in the FAM or SYBR® Green I channel (SolisGreen™ excitation maximum 489 nm, emission maximum 515 nm). It has been shown that qPCR mixes based on SolisGreen™ dye have increased performance with low target concentration demonstrating improved precision and less variance between technical replicates.
ROX (carboxy-X-rhodamine) is one of the passive reference dyes providing a constant fluorescent signal that is used for signal normalization during the amplification cycles. The emission recorded from a reference dye during the baseline cycles is used to normalize the emission recorded from the reporter (e.g. EvaGreen®, SolisGreen®, SYBR® Green, etc.) in later cycles in ROX-dependent real-time PCR systems (e.g. Applied Biosystems 5700, 7000, 7300, 7700, 7900HT, StepOne™, StepOnePlus™). Reference dye compensates for small fluorescent fluctuations and well-to-well variations that may occur.
In Solis BioDyne qPCR mixes we use technology, based on ROX, that allows us to use same mix for high- and low- ROX requiering cyclers.
Please check cycler-mix compatibility here if not sure.
Routine storage: -20 ºC
Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of the reagents.
At room temperature
Highly sensitive dye-based qPCR Mix with improved detection of low-copy targets. Compatible with most qPCR platforms.
Sensitive and highly specific cDNA synthesis and qPCR in a single tube. Suitable for ROX and no ROX cyclers.
Sensitive and highly specific cDNA synthesis and probe-based qPCR in a single tube. Ideal solution for high throughput RNA analysis.
All-inclusive and the most convenient product format for first-strand cDNA synthesis using gene-specific primers. A combined enzyme mix (SOLIScript® and RiboGrip™) and a reaction premix in separate tubes. Primers not included.
