Some applications of this product may require a license which is not provided by the purchase of this product.
For research use only.
SOLIScript® 1-step SolisGreen® Kit 2.0 is specially designed for dye-based real-time one-step RT-PCR (RT-qPCR) assays. SolisGreen® dsDNA binding dye is incorporated into the product to evaluate the DNA amplification process during qPCR. The fluorescence spectra of this dye are similar to the more widely used SYBR® Green I and is compatible with most major real-time cyclers. When compared to SYBR® Green I, SolisGreen® dye shows:
SOLIScript® 1-step SolisGreen® Kit 2.0 is an optimized ready-to-use kit that comes in 3 tubes (including nuclease free water) and contains all the necessary components, except sample (RNA template) and primers, to perform reactions with accurate and highly sensitive results. Additionally, the product includes a passive reference based on ROX dye making it compatible with both ROX-dependent and ROX-independent qPCR cyclers.
For DNA applications try out an already optimized easy-to-use HOT FIREPol® SolisGreen® qPCR Mix 2.0!
Sample type: RNA
Product format: Reagent kit (nuclease free water supplied in a separate tube)
Detection type: Dye-based, includes SolisGreen® intercalating dye
Reference dye: Based on ROX
Compatible real-time instruments: The mix is compatible with both ROX-dependent and ROX-independent qPCR cyclers. SOLIScript® 1-step SolisGreen® Kit 2.0 is not compatible with platforms that require high ROX, such as Applied BioSystems® 7900HT, StepOne™ or StepOnePlus™ systems. Check Your cycler!
40x One-step SOLIScript® Mix: SOLIScript® Reverse Transcriptase, RiboGrip® RNase Inhibitor.
5x One-step SolisGreen® Mix 2.0: HOT FIREPol® DNA polymerase, dNTPs (dATP, dCTP, dGTP, dTTP), 12.5 mM MgCl2 (1x RT-qPCR solution – 2.5 mM MgCl2), SolisGreen® dye, passive reference dye based on ROX dye.
Water, nuclease-free.
RiboGrip™ is not directly associated with tolerance to common inhibitors, however, it helps to keep RNA intact and prevent RNA degradation because RiboGrip is inactivating ribonuclease enzymes
SolisGreen™ nucleic acid dye shares spectral properties with other commonly used qPCR dyes, allowing detection in the FAM or SYBR® Green I channel (SolisGreen™ excitation maximum 489 nm, emission maximum 515 nm). SolisGreen™ is characterized by high sensitivity and great PCR efficiency with low template amounts for accurate and reproducible results.
SolisGreen™ is based on CYGREEN dye. CYGREEN is used under licence from Enzo Life Sciences, Inc. CYGREEN is a U.S. registered trademark of Enzo Life Sciences, Inc. U.S. Patent Nos. 8,153,802 and 7,569,695.
Both dyes are DNA intercalating agents that are used to stain dsDNA. SolisGreen™ dye is spectrally similar to EvaGreen® allowing detection in the FAM or SYBR® Green I channel (SolisGreen™ excitation maximum 489 nm, emission maximum 515 nm). It has been shown that qPCR mixes based on SolisGreen™ dye have increased performance with low target concentration demonstrating improved precision and less variance between technical replicates.
ROX (carboxy-X-rhodamine) is one of the passive reference dyes providing a constant fluorescent signal that is used for signal normalization during the amplification cycles. The emission recorded from a reference dye during the baseline cycles is used to normalize the emission recorded from the reporter (e.g. EvaGreen®, SolisGreen®, SYBR® Green, etc.) in later cycles in ROX-dependent real-time PCR systems (e.g. Applied Biosystems 5700, 7000, 7300, 7700, 7900HT, StepOne™, StepOnePlus™). Reference dye compensates for small fluorescent fluctuations and well-to-well variations that may occur.
In Solis BioDyne qPCR mixes we use technology, based on ROX, that allows us to use same mix for high- and low- ROX requiering cyclers.
Please check cycler-mix compatibility here if not sure.
Routine storage: -20 ºC
Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of the reagents.
At room temperature.
One-Step RT-qPCR reagent in a convenient single vial format. Includes UNG enzyme to prevent carryover contamination and false positive results. High multiplexing capacity and inhibitor tolerance enable flexible experiment design.
Highly sensitive dye-based qPCR Mix with improved detection of low-copy targets. Suitable for ROX-dependent and ROX-independent qPCR cyclers.
Sensitive and highly specific cDNA synthesis and qPCR in a single tube. Suitable for ROX and no ROX cyclers.
Sensitive and highly specific cDNA synthesis and probe-based qPCR in a single tube. Ideal solution for high throughput RNA analysis.
RiboGrip® RNase Inhibitor (220 U/µl) is an in silico-designed protein-based ribonuclease inhibitor with enhanced stability. Effective protection against RNase A, RNase B and RNase C.
A flexible and robust cDNA synthesis kit contains all the reagents you need for first-strand cDNA synthesis. Individual components enable thorough assay optimization.
A flexible and highly specific cDNA synthesis kit that contains all the reagents you need for first-strand cDNA synthesis. Individual components enable thorough assay optimization.
