Some applications of this product  may require a license which is not provided by the purchase of this product.

For research use only. 

Highly sensitive dye-based one-step RT-qPCR kit with improved detection of low-copy targets

  • cDNA synthesis and dye-based qPCR in one reaction
  • Great PCR efficiency – reliable and easy-to-analyze results
  • High sensitivity – great when working with low concentrated samples
  • Excellent fluorescence levels
  • Convenient reaction set-up at room temperature
  • Environmentally friendly – worldwide ice-free shipping

Previous version of this product, SOLIScript® 1-step SolisGreen® Kit (cat. no 08-63-XXXXX) has been discontinued. SOLIScript® 1-step SolisGreen® Kit 2.0 shows identical performance as per product specification with its previously existing product version.


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Reagents Provided
Item Pcs. Vial size
5x One-step SolisGreen® Mix 2.0 1 50 rxn | 200 µl
40x One-step SOLIScript® Mix 1 50 rxn | 25 µl
Water, nuclease free 1 1.25 ml


SOLIScript® 1-step SolisGreen® Kit 2.0 is specially designed for dye-based real-time one-step RT-PCR (RT-qPCR) assays. SolisGreen® dsDNA binding dye is incorporated into the product to evaluate the DNA amplification process during qPCR. The fluorescence spectra of this dye are similar to the more widely used SYBR® Green I and is compatible with most major real-time cyclers. When compared to SYBR® Green I, SolisGreen® dye shows:

  • higher fluorescence level
  • better sensitivity for detecting low template concentrations
  • great stability for room temperature storage

SOLIScript® 1-step SolisGreen® Kit 2.0 is an optimized ready-to-use kit that comes in 3 tubes (including nuclease free water) and contains all the necessary components, except sample (RNA template) and primers, to perform reactions with accurate and highly sensitive results. Additionally, the product includes a passive reference based on ROX dye making it compatible with both ROX-dependent and ROX-independent qPCR cyclers.


  • Gene expression analysis and absolute quantification
  • Pathogen detection and quantification
  • Low-copy gene detection
  • miRNA profiling and quantification

For DNA applications try out an already optimized easy-to-use HOT FIREPol® SolisGreen® qPCR Mix 2.0!


Sample type: RNA

Product format: Reagent kit (nuclease free water supplied in a separate tube)

Detection type: Dye-based, includes SolisGreen® intercalating dye

Reference dye: Based on ROX

Compatible real-time instruments: The mix is compatible with both ROX-dependent and ROX-independent qPCR cyclers. SOLIScript® 1-step SolisGreen® Kit 2.0 is not compatible with platforms that require high ROX, such as Applied BioSystems® 7900HT, StepOne™ or StepOnePlus™ systems. Check Your cycler!

Kit Components

40x One-step SOLIScript® Mix: SOLIScript® Reverse Transcriptase, RiboGrip® RNase Inhibitor.

5x One-step SolisGreen® Mix 2.0: HOT FIREPol® DNA polymerase, dNTPs (dATP, dCTP, dGTP, dTTP), 12.5 mM MgCl2 (1x RT-qPCR solution – 2.5 mM MgCl2), SolisGreen® dye, passive reference dye based on ROX dye.

Water, nuclease-free.

Great stability under high temperatures – worldwide ice-free shipping!
Accurate quantification across a broad dynamic range
Related Resources


Does RiboGrip help with inhibitor resistance?

RiboGrip™ is not directly associated with tolerance to common inhibitors, however, it helps to keep RNA intact and prevent RNA degradation because RiboGrip is inactivating ribonuclease enzymes

I have previously used SYBR® Green / EvaGreen® based qPCR assays. What are the spectral features of SolisGreen™ dye?

SolisGreen™ nucleic acid dye shares spectral properties with other commonly used qPCR dyes, allowing detection in the FAM or SYBR® Green I channel (SolisGreen™ excitation maximum 489 nm, emission maximum 515 nm). SolisGreen™ is characterized by high sensitivity and great PCR efficiency with low template amounts for accurate and reproducible results. 

SolisGreen™ is based on CYGREEN dye. CYGREEN is used under licence from Enzo Life Sciences, Inc. CYGREEN is a U.S. registered trademark of Enzo Life Sciences, Inc. U.S. Patent Nos. 8,153,802 and 7,569,695.

What is the difference between EvaGreen® and SolisGreen® dye?

Both dyes are DNA intercalating agents that are used to stain dsDNA. SolisGreen™ dye is spectrally similar to EvaGreen® allowing detection in the FAM or SYBR® Green I channel (SolisGreen™ excitation maximum 489 nm, emission maximum 515 nm). It has been shown that qPCR mixes based on SolisGreen™ dye have increased performance with low target concentration demonstrating improved precision and less variance between technical replicates.

Why do certain kits contain a ROX?

ROX (carboxy-X-rhodamine) is one of the passive reference dyes providing a constant fluorescent signal that is used for signal normalization during the amplification cycles. The emission recorded from a reference dye during the baseline cycles is used to normalize the emission recorded from the reporter (e.g. EvaGreen®, SolisGreen®, SYBR® Green, etc.) in later cycles in ROX-dependent real-time PCR systems (e.g. Applied Biosystems 5700, 7000, 7300, 7700, 7900HT, StepOne™, StepOnePlus™). Reference dye compensates for small fluorescent fluctuations and well-to-well variations that may occur.

In Solis BioDyne qPCR mixes we use technology, based on ROX, that allows us to use same mix for high- and low- ROX requiering cyclers. 

Please check cycler-mix compatibility here if not sure.

Storage & Shipping

Routine storage: -20 ºC

Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of the reagents.


At room temperature.