SOLIScript^® Fast 1-step RT-qPCR Mix with UNG

NEW!

Some applications of this product may require a license which is not provided by the purchase of this product.

For research use only.

Convenient single vial format and sensitive 5-plex amplification for demanding one-step RT-qPCR experiments

4x concentrated one-step RT-qPCR reagent for RNA detection
Convenient reaction setup with a single vial format
Amplify up to 5 targets within one reaction
UNG enzyme prevents carryover contamination and false positive results
Quick results thanks to fast extension rates of the in silico designed HOT SolisFAST^® DNA Polymerase
Robust performance in inhibited conditions
RNase inhibitor included in the mix

Ordering

50 rxn | 50 x 20 µl rxn free sample

200 rxn | 200 x 20 µl rxn

1000 (5 x 200 rxn) | 1000 x 20 µl rxn

5000 rxn | 5000 x 20 µl rxn

Catalog number

08-87-0000S

€ 0.00

Details

Reagents Provided

Item	Pcs.	Vial size
4x SOLIScript^®️ Fast 1-step RT-qPCR Mix with UNG	1	50 rxn \| 250 μl
Water, nuclease free	1	1.25 ml

Overview

Description

SOLIScript^® Fast 1-step RT-qPCR Mix with UNG is optimized for probe-based one-step RT-qPCR assays. It contains all components necessary in a single vial (except water, template, primers and probes) to perform cDNA synthesis and qPCR with up to 5 targets within around 1h of total reaction time. Inhibitor tolerance and compatibility with fast cycling enable flexible experiment design. Salini UNG^® Uracil-N-Glycosylase is incorporated into the product along with dUTPs to prevent carryover contamination and false positive results.

Applications

Detection and quantification of RNA and DNA targets*
DNA/LNA hydrolysis probe-based assays
Suitable for singleplex and multiplex reactions (up to 5 plex)

* Read more from the application note.

Properties

Sample type: RNA and DNA
Product format: Reagent kit (nuclease-free water supplied in a separate tube)
Detection type: Probe-based
Reference dye: none
Compatible real-time instruments: The product is compatible with most qPCR cyclers. If using a ROX-dependent platform, switch off ROX normalization prior to the run. Check your cycler!*

*Version with ROX available upon request.

Reagents

SOLIScript^® Fast 1-step RT-qPCR Mix with UNG comprises:

4x SOLIScript^® Fast 1-step RT-qPCR Mix with UNG (SOLIScript^® Reverse Transcriptase, RiboGrip^® RNase Inhibitor, HOT SolisFAST^® DNA polymerase, Salini UNG^® Uracil-N-Glycosylase, a unique reaction buffer with dNTPs (including dUTPs), 22.0 mM MgCl₂ (1x RT-qPCR solution – 5.5 mM MgCl₂) and additives to maximize PCR efficiency).
Water, nuclease-free