Properties
Sample type: RNA
Final product: cDNA (first strand)
Product format: Enzyme with buffer
Enzyme: SOLIScript® Reverse Transcriptase
Source: Purified from an E. coli strain that carries an overproducing plasmid containing a SOLIScript® Reverse Transcriptase gene.
Storage and dilution buffer: 50% glycerol (v/v), 20 mM Tris-HCl pH 7.5 at 25°C, 100 mM KCl, 0.1 mM EDTA and stabilizers.
Reagents
SOLIScript® Reverse Transcriptase
10x RT Reaction Buffer with DTT, 500 mM Tris-HCl pH 8.3, 500 mM KCl, 30 mM MgCl2, 100 mM DTT