Some applications of this product may require a license which is not provided by the purchase of this product.
For research use only.
Novel in silico-engineered, thermostable reverse transcriptase with reduced RNase H activity. SOLIScript® RT is extremely stable and will remain fully active for up to one month at room temperature.
SOLIScript® Reverse Transcriptase (RT) is an in-silico designed chimeric RNA‐directed DNA polymerase.
SOLIScript® RT can synthesize a complementary DNA strand from ssRNA or ssDNA and is active at higher temperatuures (up to 60°C).
SOLIScript® RT is a robust enzyme for RNA detection and has enhanced stability at room temperature with no activity loss for up to 1 month. This RT contains a RNase H domain with reduced activity.
Sample type: RNA
Final product: cDNA (first strand)
Product format: Enzyme with all components included reagent kit
Enzyme: SOLIScript® Reverse Transcriptase
Source: Purified from an E. coli strain that carries an overproducing plasmid containing a SOLIScript® Reverse Transcriptase gene.
Storage and dilution buffer: 50% glycerol (v/v), 20 mM Tris-HCl pH 7.5 at 25°C, 100 mM KCl, 0.1 mM EDTA and stabilizers.
SOLIScript® Reverse Transcriptase
RiboGrip® RNase Inhibitor (220 U/μl)
10x RT Reaction Buffer with DTT, 500 mM Tris-HCl pH 8.3, 500 mM KCl, 30 mM MgCl2, 100 mM DTT
Oligo (dT) Primer (100 μM)
dNTP MIX (20 mM of each)
Water, nuclease free
NB! A component of this kit has changed. RiboGrip® RNase Inhibitor (Cat. No. 06-22) has been discontinued and is replaced by a new product: RiboGrip® RNase Inhibitor 220 U/µl. Please refer to the product data sheet for up-to-date usage instructions.
RiboGrip™ is not directly associated with tolerance to common inhibitors, however, it helps to keep RNA intact and prevent RNA degradation because RiboGrip is inactivating ribonuclease enzymes
Pipetting viscous liquids like glycerol with an air displacement micropipette is difficult, because viscous liquids have a high resistance to flow. We have a few recommendations for accurate pipetting:
Solis BioDyne products should be stored at -20°C.
Shipping and temporary storage for up to 1 month at room temperature (*15−25°C) has no detrimental effects on the quality of Solis BioDyne reagents.
Freeze-thaw stability is tested for each product. Most PCR and qPCR products have passed 30 freeze-thaw cycles with no changes in performance. Specific information is found in Storage and Shipping conditions of each product.
When stored and handled under the recommended conditions, full activity of the reagents is retained until the Expiry Date printed on the tube label.
*World Health Organization (2003). Guidelines for the Storage of Essential Medicines and Other Health Commodities.
Routine storage: -20oC
Shipping and temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of the reagents.
At room temperature.
FIREScript® KIT contains a powerful genetically modified MMLV-based FIREScript® reverse transcriptase with increased thermostability and exceptional stability at room temperature. The kit format contains only the enzyme and reaction buffer allowing you to choose other components according to your preference.
A flexible and robust cDNA synthesis kit contains all the reagents you need for first-strand cDNA synthesis. Individual components enable thorough assay optimization.
All-inclusive and the most convenient product format for first-strand cDNA synthesis. A combined enzyme mix (FIREScript® and RiboGrip®) and a reaction premix in separate tubes allowing you to choose the most appropriate priming option.
SOLIScript® KIT contains a unique in silico-designed SOLIScript® reverse transcriptase with high thermostability and exceptional stability at room temperature. SOLIScript® suits best with oligo dT and gene-specific primers. The kit format contains only the enzyme and reaction buffer allowing you to choose other components according to your preference.
All-inclusive and the most convenient product format for first-strand cDNA synthesis using gene-specific primers. A combined enzyme mix (SOLIScript® and RiboGrip®) and a reaction premix in separate tubes. Primers not included.
