Some applications of this product may require a license which is not provided by the purchase of this product.
For research use only.
Solis Hot Start HiFi DNA Polymerase is a modified polymerase with 3′→5′ exonuclease (proofreading) activity demonstrating enhanced fidelity of more than 100x compared to wild type Taq polymerase, measured using single-molecule real-time (SMRT) sequencing. Chemical modification of the polymerase enables hot-start PCR that improves specificity and accuracy, minimizing mispriming and extension from non-specifically annealed primers and primer-dimers.
Suitable in applications requiring high fidelity PCR, such as sequencing or cloning. Additionally, enhanced processivity allows for long range amplification up to 12 kb from genomic DNA and 24 kb from Lambda DNA. Increased GC tolerance allows for robust amplification of challenging amplicons with GC content ranging between 30-79% when combined with the 10x GC-rich Enhancer (see figure below). Superior multiplexing capabilities ensure uniform amplifications of a diverse range of amplicons (see figure below).
Solis Hot Start HiFi DNA Polymerase is engineered to efficiently incorporate dUTPs and is well suited for amplification of bisulfite treated (bisulfite sequencing) DNA, damaged DNA (e.g., FFPE samples) or for prevention of carryover contamination (when used with dUTPs and UNG).
Additionally, the Solis Hot Start HiFi Polymerase and the whole kit is room temperature stable for up to 1 month due to the use of Stability TAG - Solis BioDyne’s proprietary and patented polypeptide stabilization technology that is incorporated into the enzyme, enabling environmentally friendly ambient temperature shipping [1]. Room temperature stability also enables maximum convenience allowing reactions to be set up without ice at room temperature.
[1] Kahre, O. et al., Compositions for increasing polypeptide stability and activity, and related methods, EP2501716B1 (2015) and US9321999B2 (2016)
Sample type: DNA
Product format: Reagent kit
Hot start: Yes, chemical (12–15-minute activation)
Fidelity: >100x compared to Taq polymerase
Detection type: end-point analysis
Overhang: Blunt
Amplicon Size: up to 12 kb complex samples (human gDNA), 24 kb low complexity DNA
Targets: 1-18+
Solis Hot Start HiFi DNA Polymerase (1 U/µl) in enzyme dilution buffer
5x HiFi Reaction Buffer with 10 mM MgCl2 (2 mM in 1x concentration)
10x GC-rich Enhancer as an additive that facilitates amplification of difficult templates (e.g., GC-rich DNA templates)
Routine storage: -20 ºC.
Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of the reagents.
At room temperature.
