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Some applications of this product  may require a license which is not provided by the purchase of this product.

For research use only. 

Superior specificity with gene-specific primers in flrxible kit format

Novel in silico-engineered, thermostable reverse transcriptase with reduced RNase H activity. SOLIScript® RT is extremely stable and will remain fully active for up to one month at room temperature.

  • superior specificity with gene-specific primers
  • wide reaction temperature 37°C - 60°C
  • reaction set-up and shipment without dry ice
  • in flexible 6-tube kit format

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Catalog number

06-35-00050

Quantity

€ 174.00

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Details

Reagents Provided
Item Pcs. Vial size
SOLIScript® Reverse Transcriptase 1 50 rxn | 50 µl
RiboGrip RNase Inhibitor 1 50 rxn | 1000 U
10x RT Reaction Buffer with DTT 1 125 µl
dNTP MIX (20 mM of each) 1 2 µmol | 25 µl
Oligo (dT) Primer 1 50 µl
Water, nuclease free 1 1.25 ml
Overview

Description

SOLIScript® Reverse Transcriptase (RT) is an in-silico designed chimeric RNA‐directed DNA polymerase.

SOLIScript® RT can synthesize a complementary DNA strand from ssRNA or ssDNA and is active at higher temperatuures (up to 60°C).

SOLIScript® RT is a robust enzyme for RNA detection and has enhanced stability at room temperature with no activity loss for up to 1 month. This RT contains a RNase H domain with reduced activity.

Applications

  • First strand cDNA synthesis
  • RT-PCR
  • RT-qPCR

Properties

Sample type: RNA

Final product: cDNA (first strand)

Product format: Enzyme with all components included reagent kit

Enzyme: SOLIScript® Reverse Transcriptase

Source: Purified from an E. coli strain that carries an overproducing plasmid containing a SOLIScript® Reverse Transcriptase gene.

Storage and dilution buffer: 50% glycerol (v/v), 20 mM Tris-HCl pH 7.5 at 25°C, 100 mM KCl, 0.1 mM EDTA and stabilizers.

Reagents

SOLIScript® Reverse Transcriptase

RiboGrip RNase Inhibitor (40 U/μl) 10x RT Reaction Buffer with DTT, 500 mM Tris-HCl pH 8.3, 500 mM KCl, 30 mM MgCl2, 100 mM DTT

Oligo (dT) Primer (100 μM)

dNTP MIX (20 mM of each)

Water, nuclease free

Superior specificity
cDNA synthesis priming options
Related Resources

FAQ

Does RiboGrip help with inhibitor resistance?

RiboGrip™ is not directly associated with tolerance to common inhibitors, however, it helps to keep RNA intact because RiboGrip inhibits Rnase H.

Enzymes are usually very viscous liquids. What would you recommend for the most accurate pipetting?

Pipetting viscous liquids like glycerol with an air displacement micropipette is difficult, because viscous liquids have a high resistance to flow. We have a few recommendations for accurate pipetting:

  • Hold the pipette vertical, since more liquid gets picked up into the tip when the pipette is held at an angle.
  • Only put the pipette tip in the liquid.
  • Use a very slow aspiration speed, wait for a few seconds until the liquid retightens and then use a very slow dispensing speed.
What are the storage conditions and stability of Solis BioDyne reagents?

Solis BioDyne products should be stored at -20°C.

Shipping and temporary storage for up to 1 month at room temperature (*1525°C) has no detrimental effects on the quality of Solis BioDyne reagents.

Freeze-thaw stability is tested for each product. Most PCR and qPCR products have passed 30 freeze-thaw cycles with no changes in performance. Specific information is found in Storage and Shipping conditions of each product.

When stored and handled under the recommended conditions, full activity of the reagents is retained until the Expiry Date printed on the tube label.

*World Health Organization (2003). Guidelines for the Storage of Essential Medicines and Other Health Commodities.

Storage & Shipping
Storage

Routine storage: -20oC


Shipping and temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of the reagents.

Shipping

At room temperature