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Some applications of this product  may require a license which is not provided by the purchase of this product.

For research use only. 

Stock solution for PCR assay optimization

25 mM MgCl2 is used for adjusting Mg2+ concentration in PCR assay.

  • additive for (q)PCR
  • additive for RT-(q)PCR

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Catalog number

05-11-00025

Quantity

€ 6.50

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Details

Overview

Description

MgCl2 solution is used for adjusting Mg2+ concentration in PCR assay. It can be used in combination with PCR buffers that come with FIREPol® and HOT FIREPol® which are all MgCl2 free). In most applications, a final concentration of 1,5 mM MgCl2 is optimal for a satisfactory yield. In some cases, MgCl2 titration is needed for a better yield and specificity.

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Storage & Shipping
Storage

Routine storage: -20ºC
Temporary storage at room temperature has no detrimental effects on the quality of this reagent.

Shipping

At room temperature