This diagnostic test helps to find how much disease is in the population to understand whether the strategies to eliminate Onchocerciasis are working or not. Unfortunately, it is not possible to completely get rid of it. There are drugs used to treat people infected by the parasite. The problem is that the drug kills parasites only in their larval state, but not the adult ones. The treatment is needed for long enough to kill the parasites. However, the larva or the adult will not necessarily harm people, they might just use people as hosts. This makes it very difficult to understand how many people might be infected since not all are exhibiting symptoms of the disease.
Mary Doherty, PhD says that it would be great to resolve the issue of neglected tropical diseases. While it is what their lab is currently focused on, they are sure they can find something else to be interested in once they have solved the current problem troubling the world.
In general, Williams’ lab loves developing new methods and testing out all kinds of new products and technologies, which could help improve how science and diagnostics are done today. It has been a pleasure working with them and we hope to continue doing that in the future.Product used: HOT FIREPol® Probe qPCR Mix Plus (ROX)
“Solis BioDyne’s HOT FIREPol® mix worked better than lyophilized alternatives that we tested. Instead of producing a master mix, lyophilizing it, and sending it, we got all from HOT FIREPol®, which made the work a lot easier,” said Mary Doherty, PhD.References:Doherty, M., Grant, J. R., Pilotte, N., Bennuru, S., Fischer, K., Fischer, P. U., Lustigman, S., Nutman, T. B., Pfarr, K., Hoerauf, A., Unnasch, T. R., Hassan, H. K., Wanji, S., Lammie, P. J., Ottesen, E., Mackenzie, C., & Williams, S. A. (2023). Optimized strategy for real-time qPCR detection of Onchocerca volvulus DNA in pooled Simulium sp. blackfly vectors. PLoS neglected tropical diseases, 17(12), e0011815. https://doi.org/10.1371/journal.pntd.0011815
Lyophilization, also known as freeze-drying, is in simple terms a water-removal process that increases product stability and preserves its functionality. Our new SolisFAST® Lyo-Ready qPCR Kit with UNG represents an optimized lyophilization-compatible qPCR solution to enhance the simplicity, convenience, and speed of diagnostic and applied testing.
The running joke with PCR is that if something can go wrong, it will go wrong. Quite often it’s even impossible to determine why some samples turned out fine while the others did not. In a situation like this, it would be amazing to know some trick or a secret to avoid spending all the time and resources to do the experiment again. Here are a few we are willing to share so that you could find love for PCR.
As an alternative to PCR, the loop-mediated isothermal amplification (LAMP) reaction has been developed for DNA detection. The LAMP test is fast, simple, and sensitive.
Another year has come to an end. It has been full of adventure, joy, and some mishaps as well since it wouldn’t be real life if everything was great all the time. Nevertheless, let’s focus on the positive to start the new year in a good mood and with a strong motivation to do even better.