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For research use only.
The 1 kb DNA Ladder is a ready-to-use molecular weight marker suitable for DNA fragment size determination on gel electrophoresis.
Solis BioDyne 1 kb DNA Ladder Ready to Load is designed for sizing and approximate quantification of double-stranded DNA in the range of 250 bp to 10,000 bp.
Solis BioDyne 1 kb DNA Ladder Ready to Load is composed of thirteen DNA fragments (in base pairs): 250, 500, 750, 1000, 1500, 2000, 2500, 3000, 4000, 5000, 6000, 8000, 10000. It contains two reference bands (1000 and 3000 bp) for easy orientation.
It contains two dyes: bromophenol blue and xylene cyanole which serve as visual aid to monitor the progress of migration during agarose gel electrophoresis.
Concentration: 0.1 µg/µl
Size range: 250 bp - 10 000 bp
No of bands: 13
Storage Solution: 10 mM EDTA, 10% glycerol, 0.015% bromophenol blue, 0.015% xylene cyanole and 0.17% SDS.
Dilute your DNA sample with the 6x DNA Loading Dye Buffer: mix 1 volume of the Loading Dye Buffer with 5 volumes of your DNA sample.
Loading Dye Buffers contain glycerol that ensures that the DNA in the ladder and sample forms a layer at the bottom of the well, and color markers (e.g. Bromophenol blue, Xylene cyanole FF, Orange G) for visual tracking of DNA migration during electrophoresis.
For the best results, use Solis BioDyne 6x Loading Dye Buffers (Blue Dye Cat. No. 07-01-00010, Double Blue Dye 07-02-00010, Orange Dye 07-04-00010, Orange Blue Dye 07-03-00010).
No. Solis Biodyne 100 bp DNA Ladder and 1 kb DNA Ladder are ready-to-use.
Solis Biodyne 100 bp DNA Ladder and 1 kb DNA Ladder are premixed with 6x DNA Loading Dye Buffer Blue (Cat. No. 07-01-00010) and 6x DNA Loading Dye Buffer Double Blue (Cat. No. 07-02-00010), respectively.
For a standard electrophoresis system, load 0.4-0.8 µl (0.04-0.08 µg) of Solis BioDyne 100 bp or 1 kb DNA Ladder Ready to Load per 1 mm of gel lane (per 1 mm of well width). It is generally recommended to load the same volumes of your DNA sample and the DNA Ladder.
A molecular-weight size marker used to identify the approximate size (usually in base pairs) and amount (usually in nanograms) of a DNA molecule run on a gel during electrophoresis, using the principle that molecular weight is inversely proportional to migration rate through a gel.
DNA ladder contains double-stranded DNA fragments whose molecule weights (and size in base pairs) are known and standardized. When run alongside your PCR product in a gel, the DNA ladder allows you to estimate the size and amount of your DNA fragment by comparing it to the closest band in the ladder lane.
Storage for up to 9 months at room temperature has no detrimental effects on the quality of this reagent.
Long term storage: -20ºC is recommended
At room temperature.
