Some applications of this product may require a license which is not provided by the purchase of this product.
For research use only.
SolisFAST® 1-step RT-PCR Kit with UNG provides a simple and streamlined workflow for analyzing RNA targets using reverse transcription PCR (RT-PCR). Both the cDNA synthesis and PCR are conveniently performed in one tube greatly simplifying the analysis workflow, reducing reaction-to-reaction variation, minimizing hands-on steps and reducing reaction set-up to results time.
Exceptionally fast and robust SolisFAST® polymerase combined with our highly processive reverse transcriptases make SolisFAST® 1-step RT-PCR Kit with UNG the fastest kit on the market to perform RT-PCR with unparalleled under 1-hour reaction times. Optimized composition ensures superior sensitivity allowing detection of as little as 0.1 pg of RNA and enabling efficient multiplexing of up to 5 RNA targets simultaneously.
Combination of our highly thermostable FIREScript® and SOLIScript® reverse transcriptases allow efficient reactions at temperatures up to 60 °C to facilitate amplifying difficult RNA targets with challenging secondary structures. The RT mix is preblended with our in silico-designed ribonuclease inhibitor RiboGrip® to minimize the risk of RNA degradation. Salini UNG® Uracil-N-Glycosylase is incorporated into the product along with dUTPs to prevent carryover contamination and false positive results.
The kit is supplied with a novel RT-PCR enhancer – AmpliBoostTM to further improve sensitivity and performance with GC-rich and other challenging amplicons.
Additionally, the kit is room temperature stable for up to 1 month due to the use of Stability TAG - Solis BioDyne’s proprietary and patented polypeptide stabilization technology that is incorporated into each enzyme, enabling environmentally friendly ambient temperature shipping [1].
[1] Kahre, O. et al., Compositions for increasing polypeptide stability and activity, and related methods, EP2501716B1 (2015) and US9321999B2 (2016)
SolisFAST® 1-step RT-PCR Kit with UNG is suitable for RT-PCR applications such as:
Sample type: RNA
Product format: reagent kit
Detection type: end-point analysis
Priming method: gene specific primers
Ready-to-load: no
Amplicon Size: up to 2 kb
Targets: 1-5 targets
4x One-step HOT SolisFAST® Mix with UNG with HOT SolisFAST® DNA polymerase, Salini UNG® Uracil-N-Glycosylase, optimized reaction buffer, dNTPs including dUTP, 6.4 mM MgCl2 (1x qPCR solution – 1.6 mM MgCl2)
40x One-step RT Mix with SOLIScript® Reverse Transcriptase, FIREScript® Reverse Transcriptase, RiboGrip® RNase Inhibitor, Salini UNG® Uracil-N-Glycosylase.
5x AmpliBoost™ RT-PCR enhancer – A novel RT-PCR enhancer to improve sensitivity and help with challenging templates (e.g. GC-rich, secondary RNA structures).
Water, nuclease free
Routine storage at -20 °C.
Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of the reagents.
At room temperature.
Sensitive and highly specific cDNA synthesis and end-point PCR in a single tube. Ready-to-Load version to streamline your gel electrophoresis workflow.
