Some applications of this product may require a license which is not provided by the purchase of this product.
For research use only.
SoliSD™ Lyo-compatible Bsm DNA Polymerase Kit is provided in a flexible 5-vial format. Implemented Stability TAG and the unique SoliSD™ Supplement system improves the performance of the enzyme:
SoliSD™ Glycerol-Free Bsm DNA Polymerase is based on the Large Fragment of the DNA Polymerase from the genus Bacillus smithii with functional similarity to other Bst and Bsm DNA polymerases. Implemented Stability TAG technology [1] ensures incredible stability for at least 1 month at 37°C.
SoliSD™ Lyo-compatible Bsm DNA Polymerase Kit is provided in a 5-vial format, where composition gives flexibility for developing your own isothermal amplification assay. The unique SoliSD™ Supplement system is provided with the kit. Technology improves the performance of the SoliSD™ Bsm DNA Polymerase:
The enzyme has a strong strand displacement DNA polymerase activity and lacks 5’→3’ and 3’→5’ exonuclease activities. It is designed for applications requiring synthesis through double-stranded DNA regions, such as loop-mediated isothermal amplification (LAMP).
[1] Kahre, O. et al., Compositions for increasing polypeptide stability and activity, and related methods, EP2501716B1 (2015) and US9321999B2 (2016).
Lyophilization series:
To protect the biological material, such as enzymes, from the temperature and pressure changes during lyophilization, freeze-drying protectants are employed. These protecting excipients ensure that the materials reserve their active state after lyophilisation. In addition to that, they provide an adequate structure to the lyophilized cake or bead.
Lyophilization, commonly known as freeze-drying, is a process employed to remove the water content from a substance by sublimation after it has been frozen, while preserving its structure and properties. The reagents can be lyophilized to a spheric lyo-bead or a compact lyo-cake format.
Lyophilization helps to extend the product’s shelf life, enhance the stability of the reagents at higher tempereatures, even when mixed together with primers and probes, and allows for convenient and accurate dispensing of the reagents. After lyophilization, the reagents are easily reconstituted with sample material.
Routine storage at -20 °C.
At room temperature.
Exceptionally stable SoliSD™ Bsm DNA Polymerase for isothermal amplification.
In the new SoliSD™ Lyo-compatible RT-LAMP Kit all enzymes are compatible with freeze drying and contain the Stability TAG.
