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SOLIScript® SARS-CoV-2 RT-qPCR Multiplex Assay Kit (IVD)

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For sensitive and reliable detection of SARS-CoV-2 by one-step RT-qPCR

The SOLIScript® SARS-CoV-2 RT-qPCR Multiplex Assay Kit is an in vitro qualitative diagnostic test intended for the detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) genetic material in biological samples obtained from the upper respiratory tract (nasopharyngeal swabs).

The SOLIScript® SARS-CoV-2 RT-qPCR Multiplex Assay Kit is designed for simultaneous detection of three distinct regions in SARS-CoV-2 genome: Nucleocapsid gene (N), Envelope gene (E) and RNA-dependent RNA polymerase gene (RdRP), as well as human RNase P transcript (RPP30).

Highly specific and sensitive assay design enables accurate detection of all circulating SARS-CoV-2 Variants of Concern (VOC) and Variants of Interest (VOI), including Delta (B.1.617.2) and Omicron (BA.1, BA.2, BA.4, BA.5).

Read more under product description. 

Ordering

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Catalog number

08-85-00100

Quantity

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Details

Reagents Provided
Item Pcs. Vial size
Enzyme Mix 1 1 100 rxn | 440 µl
Enzyme Mix 2 1 100 rxn | 55 µl
Primer/Probe Mix 1 100 rxn | 110 μl
Positive control 1 80 μl
Nuclease-free Water 1 1.25 ml
Overview

Description

SOLIScript SARS-CoV-2 RT-qPCR Multiplex Assay Kit is an in vitro qualitative diagnostic test for multiplex detection of three distinct regions of SARS-CoV-2 genome (N, E, RdRP) along with an internal control (human Rnase P transcript) from nasopharyngeal swab samples.

  • Excellent sensitivity and precision (LoD of 10 copies per reaction).
  • Clinical sample testing presented 100% concordance when compared to validated reference assay (45/45 positive and 24/24 negative).
  • No cross-reactivity with SARS-CoV-2 near-neighbour species/strains and other respiratory pathogens.
  • Accurate detection of all circulating SARS-CoV-2 Variants of Concern (VOC) and Variants of Interest (VOI) including Delta (B.1.617.2) and Omicron (BA.1, BA.2, BA.4, BA.5).
  • Fast turnaround time after RNA extraction: BioRad CFX96 one-step RT-qPCR test results in one hour.
  • No background amplification from genomic DNA by use of a RNase P assay detecting exclusively mRNA transcripts.

The kit is intended for use exclusively by qualified and trained professionals in a diagnostic laboratory with the appropriate equipment and safety standards.

Properties

Sample type: RNA

Product format: Reagent kit with primers and probes for SARS-CoV-2 detection

Detection type: Probe-based

Reference dye: not included 

Regulatory status: IVD

Compatible real-time instruments: BioRad CFX96, ABI 7500, QS5, Roche LC480, Rotor-Gene Q.

Reagents 

Enzyme Mix 1 HOT FIREPol® DNA polymerase, reaction buffer, dNTPs

Enzyme Mix 2 SOLIScript® Reverse Transcriptase, RiboGrip® RNase Inhibitor

Primer/Probe Mix Primers and fluorescently labelled probes for SARS-CoV-2 regions in N (FAM), E (HEX), RdRP (ROX) genes, human RNase P mRNA (Cy5)

Positive Control dsDNA Positive Control containing targets specific to the SARS-CoV-2 genomic regions N, E and RdRP targeted by the assay

Nuclease-free Water RNAse/DNAse free PCR grade water

Related Resources
Storage & Shipping
Storage

Routine storage at -20°C until Expiry Date.

The kit can be stored at +4°C for up to one month.

Shipping

On blue ice or at 2°C to 8°C.