The SOLIScript® SARS-CoV-2 RT-qPCR Multiplex Assay Kit is an in vitro qualitative diagnostic test intended for the detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) genetic material in biological samples obtained from the upper respiratory tract (nasopharyngeal swabs).
The SOLIScript® SARS-CoV-2 RT-qPCR Multiplex Assay Kit is designed for simultaneous detection of three distinct regions in SARS-CoV-2 genome: Nucleocapsid gene (N), Envelope gene (E) and RNA-dependent RNA polymerase gene (RdRP), as well as human RNase P transcript (RPP30).
Highly specific and sensitive assay design enables accurate detection of all circulating SARS-CoV-2 Variants of Concern (VOC) and Variants of Interest (VOI), including Delta (B.1.617.2) and Omicron (BA.1, BA.2, BA.4, BA.5).
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SOLIScript SARS-CoV-2 RT-qPCR Multiplex Assay Kit is an in vitro qualitative diagnostic test for multiplex detection of three distinct regions of SARS-CoV-2 genome (N, E, RdRP) along with an internal control (human Rnase P transcript) from nasopharyngeal swab samples.
The kit is intended for use exclusively by qualified and trained professionals in a diagnostic laboratory with the appropriate equipment and safety standards.
Sample type: RNA
Product format: Reagent kit with primers and probes for SARS-CoV-2 detection
Detection type: Probe-based
Reference dye: not included
Regulatory status: IVD
Compatible real-time instruments: BioRad CFX96, ABI 7500, QS5, Roche LC480, Rotor-Gene Q.
Enzyme Mix 1 HOT FIREPol® DNA polymerase, reaction buffer, dNTPs
Enzyme Mix 2 SOLIScript® Reverse Transcriptase, RiboGrip™ RNase Inhibitor
Primer/Probe Mix Primers and fluorescently labelled probes for SARS-CoV-2 regions in N (FAM), E (HEX), RdRP (ROX) genes, human RNase P mRNA (Cy5)
Positive Control dsDNA Positive Control containing targets specific to the SARS-CoV-2 genomic regions N, E and RdRP targeted by the assay
Nuclease-free Water RNAse/DNAse free PCR grade water
Routine storage at -20°C until Expiry Date.
The kit can be stored at +4°C for up to one month.
On blue ice or at 2°C to 8°C.
SOLIScript® SARS-CoV-2 RT-qPCR Multiplex Assay Kit 2.0 is a multiplex assay optimized for RT-qPCR detection of SARS-CoV-2 viral RNA extracted from clinical samples or extracted from any other sample containing SARS-CoV-2 viral RNA. Includes primers and positive control.
An optimized formulation for SARS-CoV-2 RNA detection from RNA samples extracted from swab specimens. The Kit enables the users to develop their own assays based on US CDC and WHO-listed primer and probe sequences to detect SARS-CoV-2, as well as develop custom multi-target tests.