SOLIScript® SARS-CoV-2 RT-qPCR Multiplex Assay Kit 2.0 (RUO)

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Some applications of this product  may require a license which is not provided by the purchase of this product.

For research use only. 

Full RUO kit - highly sensitive for detecting all variants of concern including Omicron

The SOLIScript® SARS-CoV-2 RT-qPCR Multiplex Assay Kit 2.0 is optimized for one-step RT-qPCR detection of SARS-CoV-2 viral RNA in RNA samples extracted from nasopharyngeal swabs, bronchoalveolar lavage, sputum, or any other respiratory sample.

The kit is designed for multiplex detection of three distinct regions in the SARS-CoV-2 genome - the Nucleocapsid (N), Envelope (E) and RNA-dependent RNA polymerase (RdRP) genes; and the internal control - the human RNase P transcript (RPP30), all in a single reaction

  • High specificity – targets 3 highly conserved regions of SARS-CoV-2 genome.
  • Great analytical sensitivity – the limit of detection (LoD) of 5-10 viral RNA copies per reaction.
  • Tested and confirmed in clinical setting.
  • Suitable for screening – pooling of up to 5 samples without loss in sensitivity.
  • Excellent for high throughput workflow.
  • Fast results – one-step RT-qPCR test results in one hour.
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Catalog number

08-83-00100

Quantity

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Details

Reagents Provided
Item Pcs. Vial size
40x One-step SOLIScript® CoV Mix 1 100 rxn | 50 µl
5x One-step Probe CoV Mix 1 100 rxn | 400 µl
20x SARS-CoV-2 Primer/Probe Mix (N/E/RdRP/RP) 1 100 rxn | 100 μl
SARS-CoV-2 Positive Control (N/E/RdRP) 1 80 μl
Water, nuclease free 1 1.25 ml
Overview

Description

SOLIScript® SARS-CoV-2 RT-qPCR Multiplex Assay Kit 2.0 is a multiplex assay optimized for RT-qPCR detection of SARS-CoV-2 viral RNA extracted from clinical samples or extracted from any other sample containing SARS-CoV-2 viral RNA. Clinical specimen processing should be performed in accordance with pertaining national biological safety regulations and following the recommended World Health Organization (WHO) guidelines on biosafety and biosecurity. The kit is based on SOLIScript® 1-step CoV Kit .

SOLIScript® SARS-CoV-2 RT-qPCR Multiplex Assay Kit 2.0 is designed for simultaneous detection of three distinct regions in SARS-CoV-2 genome: Nucleocapsid gene (N, detected in FAM channel), Envelope gene (E, VIC/HEX/JOE channel) and RNA-dependent RNA polymerase gene (RdRP, Cal Red 610/ROX/Texas Red channel), as well as human RNase P transcript (RPP30, Quasar 670/Cy5 channel). 

Human RNase P primers and probe set is designed to detect exclusively mRNA transcripts (genomic DNA is not amplified) and serves as an internal control to monitor sample quality and RT-qPCR amplification.

The kit includes the proprietary in-silico designed SOLIScript® Reverse Transcriptase characterized by enhanced ability to tolerate higher temperature (up to 65oC). It also contains an RNase Inhibitor RiboGripTM to protect RNA samples from degradation. HOT FIREPol® DNA polymerase is a chemically blocked hot-start polymerase activated by a 10 min incubation step at 95°C.

Properties

Sample type: RNA

Product format: Reagent kit with primers and probes for SARS-CoV-2 detection

Detection type: Probe-based

Reference dye: not included 

Regulatory status: RUO - Research Use Only

Compatible real-time instruments: Most qPCR platforms.

Reagents 

40x One-step SOLIScript® CoV Mix with SOLIScript® Reverse Transcriptase and RiboGrip™ RNase Inhibitor 

5x One-step Probe CoV Mix  with HOT FIREPol® DNA polymerase, reaction buffer, dNTPs, 15 mM MgCl2 (1x qPCR solution - 3 mM MgCl2)

20x SARS-CoV-2 Primer/Probe Mix (N/E/RdRP/RP) designed for SARS-CoV-2 regions in N (FAM channel), E (VIC/HEX/JOE channel), RdRP (Cal Red 610/ROX/Texas Red channel) genes, as well as human RNase P mRNA transcripts (Quasar 670/Cy5 channel)

SARS-CoV-2 positive control (N/E/RdRP)

Water, nuclease free

Figure1. Multiplex Assay Kit targets
Figure 2. Performance validated using clinical samples
Figure 3. Highly sensitive detection of three viral targets with LoD 5-10 copies/reaction
Table 1. Clinical agreement study
Related Resources
Storage & Shipping
Storage

Routine storage at -20°C until Expiry Date

The kit can be stored at +4°C for up to one month

Shipping

On blue ice or at 2°C to 8°C