Some applications of this product may require a license which is not provided by the purchase of this product.
For research use only.
The SOLIScript® SARS-CoV-2 RT-qPCR Multiplex Assay Kit 2.0 is optimized for one-step RT-qPCR detection of SARS-CoV-2 viral RNA in RNA samples extracted from nasopharyngeal swabs, bronchoalveolar lavage, sputum, or any other respiratory sample.
The kit is designed for multiplex detection of three distinct regions in the SARS-CoV-2 genome - the Nucleocapsid (N), Envelope (E) and RNA-dependent RNA polymerase (RdRP) genes; and the internal control - the human RNase P transcript (RPP30), all in a single reaction
SOLIScript® SARS-CoV-2 RT-qPCR Multiplex Assay Kit 2.0 is a multiplex assay optimized for RT-qPCR detection of SARS-CoV-2 viral RNA extracted from clinical samples or extracted from any other sample containing SARS-CoV-2 viral RNA. Clinical specimen processing should be performed in accordance with pertaining national biological safety regulations and following the recommended World Health Organization (WHO) guidelines on biosafety and biosecurity. The kit is based on SOLIScript® 1-step CoV Kit .
SOLIScript® SARS-CoV-2 RT-qPCR Multiplex Assay Kit 2.0 is designed for simultaneous detection of three distinct regions in SARS-CoV-2 genome: Nucleocapsid gene (N, detected in FAM channel), Envelope gene (E, VIC/HEX/JOE channel) and RNA-dependent RNA polymerase gene (RdRP, Cal Red 610/ROX/Texas Red channel), as well as human RNase P transcript (RPP30, Quasar 670/Cy5 channel).
Human RNase P primers and probe set is designed to detect exclusively mRNA transcripts (genomic DNA is not amplified) and serves as an internal control to monitor sample quality and RT-qPCR amplification.
The kit includes the proprietary in-silico designed SOLIScript® Reverse Transcriptase characterized by enhanced ability to tolerate higher temperature (up to 65oC). It also contains an RNase Inhibitor RiboGrip® to protect RNA samples from degradation. HOT FIREPol® DNA polymerase is a chemically blocked hot-start polymerase activated by a 10 min incubation step at 95°C.
Sample type: RNA
Product format: Reagent kit with primers and probes for SARS-CoV-2 detection
Detection type: Probe-based
Reference dye: not included
Regulatory status: RUO - Research Use Only
Compatible real-time instruments: Most qPCR platforms.
40x One-step SOLIScript® CoV Mix with SOLIScript® Reverse Transcriptase and RiboGrip® RNase Inhibitor
5x One-step Probe CoV Mix with HOT FIREPol® DNA polymerase, reaction buffer, dNTPs, 15 mM MgCl2 (1x qPCR solution - 3 mM MgCl2)
20x SARS-CoV-2 Primer/Probe Mix (N/E/RdRP/RP) designed for SARS-CoV-2 regions in N (FAM channel), E (VIC/HEX/JOE channel), RdRP (Cal Red 610/ROX/Texas Red channel) genes, as well as human RNase P mRNA transcripts (Quasar 670/Cy5 channel)
SARS-CoV-2 positive control (N/E/RdRP)
Water, nuclease free
Routine storage at -20°C until Expiry Date
The kit can be stored at +4°C for up to one month
On blue ice or at 2°C to 8°C
The SOLIScript® SARS-CoV-2 RT-qPCR Multiplex Assay Kit is an in vitro qualitative diagnostic test intended for the detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) genetic material in biological samples obtained from the upper respiratory tract(nasopharyngeal swabs).
An optimized formulation for SARS-CoV-2 RNA detection from RNA samples extracted from swab specimens. The Kit enables the users to develop their own assays based on US CDC and WHO-listed primer and probe sequences to detect SARS-CoV-2, as well as develop custom multi-target tests.
