8755_kit_08-67-00250_CoV.jpg
NEW!

Some applications of this product  may require a license which is not provided by the purchase of this product.

For research use only. 

Sensitive and highly specific 1-step RT-qPCR kit optimized for SARS-CoV-2 detection

Convenient kit format for performing highly specific cDNA synthesis and probe-based qPCR in a single tube. The kit contains qPCR mix, SOLIScript® RT enzyme mix with RiboGrip™ RNase inhibitor, and nuclease-free water.

  • Optimized for SARS-CoV-2 detection
  • Flexible assay set-up
  • Suitable for multiplexing (up to 4 targets in a single assay)
  • Reaction set-up and shipment without ice
  • RNase inhibitor included in the kit

Ordering

Choose Product Size
Catalog number

08-65-00250

Quantity

Request a quote

Details

Reagents Provided
Item Pcs. Vial size
40x One-step SOLIScript® CoV Mix 1 250 rxn | 125 µl
5x One-step Probe CoV Mix 1 250 rxn | 1 ml
Water, nuclease free 1 5 ml
Overview

Description

SOLIScript® 1-step CoV Kit is optimized for highly sensitive detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) viral RNA by one-step real-time RT-PCR (RT-qPCR).

The Kit is suitable for the detection of up to four targets simultaneously and can be used with all common qPCR cyclers. Follow recommendations specific for a selected SARS-CoV-2 diagnostic panel, and instrument-specific guidelines. Contact us if a passive reference dye is required for signal normalization.

SARS-CoV-2 primers and probes, applicable positive and internal extraction controls are not provided with the Kit and should be supplied by the user.

SOLIScript® 1-step CoV Kit is tested and validated for SARS-CoV-2 detection by diagnostic laboratories. Please see our recommendations for US-CDC and Berlin-Charité protocols. 

Applications

Detection and quantification of RNA targets with qPCR (including SARS-CoV-2)

DNA/LNA hydrolysis probe-based assays

Suitable for singleplex and multiplex reactions

Properties

Sample type: RNA

Product format: Reagent kit 

Detection type: Probe-based

Reference dye: not included 

Compatible real-time instruments: Most qPCR platforms. Check Your cycler!

Reagents 

5x One-step Probe CoV Mix  with HOT FIREPol® DNA polymerase, reaction buffer, dNTPs, 15 mM MgCl2 (1x qPCR solution - 3 mM MgCl2)

40x One-step SOLIScript® CoV Mix with SOLIScript® Reverse Transcriptase and RiboGrip™ RNase Inhibitor 

Water, nuclease free

Performance validated with clinical samples
Related Resources

FAQ

Does RiboGrip help with inhibitor resistance?

RiboGrip™ is not directly associated with tolerance to common inhibitors, however, it helps to keep RNA intact because RigoGrip inhibits Rnase H.

Which primes, probes are recommended for COVID-19 detection with Your 1-step kit?

The Kit has been tested and validated by diagnostic laboratories using primer-probe panels recommended by Charité, (Germany) and CDC (USA) protocols according to WHO “Coronavirus disease (COVID-19) technical guidance: Laboratory testing for 2019-nCoV in humans”, section: “Molecular assays to diagnose COVID-19”; subsection: “In-house developed molecular assays”

Are primers, probes and positive control included in the 1-step RT-qPCR CoV kit?

SARS-CoV-2 primers and probes, applicable positive and internal extraction controls are not provided with the Kit and should be supplied by the user.

Why do certain kits contain a ROX?

ROX (carboxy-X-rhodamine) is one of the passive reference dyes providing a constant fluorescent signal that is used for signal normalization during the amplification cycles. The emission recorded from a reference dye during the baseline cycles is used to normalize the emission recorded from the reporter (e.g. EvaGreen®, SolisGreen®, SYBR® Green, etc.) in later cycles in ROX-dependent real-time PCR systems (e.g. Applied Biosystems 5700, 7000, 7300, 7700, 7900HT, StepOne™, StepOnePlus™). Reference dye compensates for small fluorescent fluctuations and well-to-well variations that may occur.

In Solis BioDyne qPCR mixes we use technology, based on ROX, that allows us to use same mix for high- and low- ROX requiering cyclers. 

Please check cycler-mix compatibility here if not sure.

Storage & Shipping
Storage

Routine storage: -20oC

Temporary storage for up to 30 days at room temperature has no detrimental effects on the quality of SOLIScript® 1-step CoV Kit

Shipping

At room temperature