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Some applications of this product  may require a license which is not provided by the purchase of this product.

For research use only. 

Sensitive and highly specific 1-step RT-qPCR reagents optimized for SARS-CoV-2 detection

Convenient kit format for performing highly specific cDNA synthesis and probe-based qPCR in a single tube. The kit contains qPCR mix, SOLIScript® RT enzyme mix with RiboGrip™ RNase inhibitor, and nuclease-free water.

  • Optimized for SARS-CoV-2 detection
  • Flexible assay set-up
  • Suitable for multiplexing (up to 4 targets in a single assay)
  • Reaction set-up and shipment without ice
  • RNase inhibitor included in the kit

Ordering

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Catalog number

08-65-00250

Quantity

€ 450.00

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Details

Reagents Provided
Item Pcs. Vial size
40x One-step SOLIScript® CoV Mix 1 250 rxn | 125 µl
5x One-step Probe CoV Mix 1 250 rxn | 1 ml
Water, nuclease free 1 5 ml
Overview

Description

SOLIScript® 1-step CoV Kit is optimized for highly sensitive detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) viral RNA by one-step real-time RT-PCR (RT-qPCR).

The Kit is suitable for the detection of up to four targets simultaneously and can be used with all common qPCR cyclers. Follow recommendations specific for a selected SARS-CoV-2 diagnostic panel, and instrument-specific guidelines. Contact us if a passive reference dye is required for signal normalization.

SARS-CoV-2 primers and probes, applicable positive and internal extraction controls are not provided with the Kit and should be supplied by the user.

SOLIScript® 1-step CoV Kit is tested and validated for SARS-CoV-2 detection by diagnostic laboratories. Please see our recommendations for US-CDC and Berlin-Charité protocols. 

Note! Amplification of more than one target in a single reaction may require considerable optimization, individual assays must be validated prior to multiplexing. For multiplex reactions, design primer pairs with similar annealing temperatures for all targets to be amplified. Self-complementarity of primers should be avoided.

Applications

Detection and quantification of RNA targets with qPCR (including SARS-CoV-2)

DNA/LNA hydrolysis probe-based assays

Suitable for singleplex and multiplex reactions

Properties

Sample type: RNA

Product format: Reagent kit 

Detection type: Probe-based

Reference dye: not included 

Compatible real-time instruments: Most qPCR platforms. Check Your cycler!

Reagents 

5x One-step Probe CoV Mix  with HOT FIREPol® DNA polymerase, reaction buffer, dNTPs, 15 mM MgCl2 (1x qPCR solution - 3 mM MgCl2)

40x One-step SOLIScript® CoV Mix with SOLIScript® Reverse Transcriptase and RiboGrip™ RNase Inhibitor 

Water, nuclease free

Performance validated with clinical samples
Highly sensitive detection, evaluated with SARS-CoV-2 synthetic single stranded RNA
Related Resources

FAQ

Does RiboGrip help with inhibitor resistance?

RiboGrip™ is not directly associated with tolerance to common inhibitors, however, it helps to keep RNA intact because RiboGrip inhibits Rnase H.

Which primes, probes are recommended for COVID-19 detection with Your 1-step kit?

The Kit has been tested and validated by diagnostic laboratories using primer-probe panels recommended by Charité, (Germany) and CDC (USA) protocols according to WHO “Coronavirus disease (COVID-19) technical guidance: Laboratory testing for 2019-nCoV in humans”, section: “Molecular assays to diagnose COVID-19”; subsection: “In-house developed molecular assays”

Are primers, probes and positive control included in the 1-step RT-qPCR CoV kit?

SARS-CoV-2 primers and probes, applicable positive and internal extraction controls are not provided with the Kit and should be supplied by the user.

Why do certain kits contain a ROX?

ROX (carboxy-X-rhodamine) is one of the passive reference dyes providing a constant fluorescent signal that is used for signal normalization during the amplification cycles. The emission recorded from a reference dye during the baseline cycles is used to normalize the emission recorded from the reporter (e.g. EvaGreen®, SolisGreen®, SYBR® Green, etc.) in later cycles in ROX-dependent real-time PCR systems (e.g. Applied Biosystems 5700, 7000, 7300, 7700, 7900HT, StepOne™, StepOnePlus™). Reference dye compensates for small fluorescent fluctuations and well-to-well variations that may occur.

In Solis BioDyne qPCR mixes we use technology, based on ROX, that allows us to use same mix for high- and low- ROX requiering cyclers. 

Please check cycler-mix compatibility here if not sure.

Storage & Shipping
Storage

Routine storage: -20oC

Temporary storage for up to 30 days at room temperature has no detrimental effects on the quality of SOLIScript® 1-step CoV Kit

Shipping

At room temperature