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For research use only.
RiboGrip®️ Glycerol-Free RNase Inhibitor (220 U/µl) is an in silico-designed protein-based ribonuclease inhibitor, which inactivates RNase A, RNase B and RNase C.
RiboGrip® inhibits the activity of ribonuclease A by noncovalently binding in a non-competitive mode at a ratio of 1:1. It is primarily used to prevent RNA degradation by contaminating RNases in various assays that use RNA sample materials, such as first-strand cDNA synthesis, RT-(q)PCR, RT-LAMP, etc.
RiboGrip® also includes a genetic modification - Stability TAG - Solis BioDyne’s proprietary and patented polypeptide stabilization technology [1]. Stability TAG makes RiboGrip® extremely tolerant to higher temperatures and enables room temperature shipping as well as effective use in assays requiring high incubation temperatures.
Highly concentrated 220 U/µl and glycerol-free formulation ensures compatibility with lyophilization and air-drying. This product does not contain excipients, please add your own as required or contact us for more information.
[1] Kahre, O. et al., Compositions for increasing polypeptide stability and activity, and related methods, EP2501716B1 (2015) and US9321999B2 (2016)
Compatible with lyophilisation and air-drying. Preventing RNA degradation in a variety of applications including:
Concentration: 220 U/µl
Origin: Recombinant mammalian chimeric protein
Source: Purified from an E. coli strain that carries an overproducing plasmid containing a RiboGrip® RNase Inhibitor gene.
Molecular weight: 47 kD monomer
Unit definition: One unit is defined as the amount of protein required to inhibit the activity of 5 ng of RNAse A by 50%. Activity is measured by the inhibition of hydrolysis of cytidine 2',3'‐cyclic monophosphate by RNase A.
Storage buffer: 25 mM Tris-HCl pH 7.5, 100 mM KCl, 0.1 mM EDTA, 5 mM DTT and stabilizers.
Specificity: RNase A, RNase B, RNase C
Recommendations for use: 0,5-2.5 U/μl final reaction concentration
Routine storage: -20ºC.
Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of the reagents.
At room temperature.
RiboGrip® RNase Inhibitor (220 U/µl) is an in silico-designed protein-based ribonuclease inhibitor with enhanced stability. Effective protection against RNase A, RNase B and RNase C.
