Some applications of this product may require a license which is not provided by the purchase of this product.
For research use only.
HOT SolisAcura™ Cassette-Based Genotyping Mix (ROX) is based on genetically modified HOT SolisAcura™ Exo(-) DNA Polymerase, giving the master mix a feature of detecting misaligned nucleotide at the 3’ ends of primer, resulting in superior performance in SNP detection and allele discrimination. It has 10-15x higher fidelity compared to wild-type Taq polymerase, which comes from choosing nucleotides more accurately. Additionally, there is no 3′→5′ exonuclease (proofreading) activity with this enzyme.
The novel polymerase is also engineered to have faster synthesis rates and is inherently inhibitor tolerant, ensuring excellent performance with crude or challenging samples. It comes with chemical hot-start that improves the specificity and accuracy of the reaction, minimizes mispriming and extension from non-specifically annealed primers and primer-dimers.
This enzyme is suitable for SNP detection via allele-specific PCR, using bi-allelic discrimination that is achieved with a universal reverse primer and two competitively binding allele-specific forward primers, each with a unique tail sequence that corresponds to either of two universal FRET cassettes. HOT SolisAcura™ Cassette-Based Genotyping Mix (ROX) is also compatible with KASP™, PACE® and Amplifluor® marker assays.
HOT SolisAcura™ Cassette-Based Genotyping Mix (ROX) is room temperature stable for up to 1 month due to the use of Stability TAG – Solis BioDyne’s proprietary and patented polypeptide stabilization technology that is incorporated into the enzyme, enabling environmentally friendly ambient temperature shipping [1]. Room temperature stability also enables maximum convenience allowing reactions to be set up without ice at room temperature.
[1] Kahre, O. et al., Compositions for increasing polypeptide stability and activity, and related methods, EP2501716B1 (2015) and US9321999B2 (2016)
Trademark information: KASP™ is a trademark of LGC Biosearch Technologies. PACE® is a registered trademark of 3CR Bioscience. Amplifluor® is a registered trademark of Merck KGaA.
Concentration: 2X
Sample type: DNA
Product format: Master mix
Hot start: Yes, chemical (15-minute activation)
Fidelity: 10-15x compared to Taq polymerase
Targets: 2 alleles
Routine storage: -20 ºC.
Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of the reagents.
At room temperature.
Novel DNA polymerase with exceptional accuracy, mismatch discrimination and inhibitor tolerance for a variety of allele-specific and precision-driven applications.
Probe genotyping mastermix based on a novel SolisAcura™ Exo(+) DNA polymerase with increased limit of detection for allele discrimination.
This master mix has been developed for TaqMan® hydrolysis probes but is also suitable for other hydrolysis probe types.
