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Some applications of this product  may require a license which is not provided by the purchase of this product.

For research use only. 

Fast, highly sensitive and reproducible probe-based qPCR solution

Extremely fast and highly sensitive probe-based qPCR Mix that has
been optimized for detection and quantification of up to five targets
simultaneously

  • delivers qPCR results 2x faster (in as little as 30 minutes)
  • reliable quantification in up to 5-plex assays
  • consistent results with low- and high-copy targets
  • robust performance
  • 30-day stability at room temperature

Ordering

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Catalog number

28-02-00001

Quantity

€ 174.00

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Details

Overview

Description

SolisFAST® Probe qPCR Mix is a 5x-concentrated, ready-to-use solution for fast, highly sensitive and reproducible probe-based qPCR assays using dual-labeled hydrolysis probes (e.g. TaqMan® probes), and is suitable for detection and quantitation of up to five targets simultaneously.

Combining our novel in silico- designed SolisFAST® DNA Polymerase and optimized buffer, this mix offers robust qPCR, and accurate target detection combined with ice free shipping and reaction set-up

Applications

  • DNA/LNA hydrolysis probe based assays
  • Detection and quantification of DNA and cDNA targets
  • Profiling gene expression
  • Microbial detection
  • Viral load determination

Properties

Concentration: 5x

Hot-start: yes, 3 minutes initial incubation at 95°C

Cycling speed: fast and ultra-fast

Detection type: Probe-based

Reference dye: ROX

Multiplex: up to 5 plexes simultaneously

Compatible real-time instruments: The mix is compatible with qPCR cyclers that require an internal reference dye (e.g. ROX) for normalization of fluorescent signal. Check Your cycler!

Mix Components:

SolisFAST® DNA PolymeraseIn silico- designed analogue of Taq DNA polymerase with enhanced stability at room temperature, fast hot-start and faster extension rates compared to the wild-type Taq DNA polymerase.

qPCR buffer: Includes 15 mM MgCl2 (1x PCR solution 3 mM MgCl2), dNTPs (dATP, dCTP, dGTP, dTTP), additives that maximize efficiency of PCR

Reference dye: ROX dye is an internal passive reference dye used to normalize the fluorescent reporter signal generated in qPCR

Excellent for multiplex assays
Accurate and sensitive qPCR
2x less time from samples to results
Related Resources

FAQ

What is the difference between dye-based and probe-based systems?

Dye-based detection (e.g., EvaGreen®, SolisGreen®, SYBR® Green) is a cost-effective qPCR option, as it requires only addition of PCR primers. However, the intercalating dye will detect any dsDNA (non-specific amplicons, primer dimers) produced in the reaction. Melt curve analysis performed after the qPCR can be used to verify the specificity of amplification and to check for the presence of non-specific amplification products.

The probe-based qPCR system demonstrates higher specificity compared to dye-based qPCR, because probes only detect the gene of interest. Keep in mind that in probe-based assays, primer dimers and non-specific products will not be detected, however, they may compromise the PCR efficiency. Using probe-based qPCR system, it is possible to distinguish between sequences with high similarity (e.g. single-nucleotide variations). Additionally, probe-based qPCR assays allow for multiplex reactions in one tube, while only a single target can be amplified and measured in a dye-based qPCR.

After first-strand cDNA synthesis, how much cDNA template should I use for my PCR?

Recommended final amount of cDNA sample in downstream PCR reaction is up to one tenth of the final reaction volume. Overload of cDNA sample may compromise the downstream PCR, because cDNA sample may contain reaction components that may inhibit your PCR reaction.

Why do certain kits contain a ROX?

ROX (carboxy-X-rhodamine) is one of the passive reference dyes providing a constant fluorescent signal that is used for signal normalization during the amplification cycles. The emission recorded from a reference dye during the baseline cycles is used to normalize the emission recorded from the reporter (e.g. EvaGreen®, SolisGreen®, SYBR® Green, etc.) in later cycles in ROX-dependent real-time PCR systems (e.g. Applied Biosystems 5700, 7000, 7300, 7700, 7900HT, StepOne™, StepOnePlus™). Reference dye compensates for small fluorescent fluctuations and well-to-well variations that may occur.

In Solis BioDyne qPCR mixes we use technology, based on ROX, that allows us to use same mix for high- and low- ROX requiering cyclers. 

Please check cycler-mix compatibility here if not sure.

What are the storage conditions and stability of Solis BioDyne reagents?

Solis BioDyne products should be stored at -20°C.

Shipping and temporary storage for up to 1 month at room temperature (*1525°C) has no detrimental effects on the quality of Solis BioDyne reagents.

Freeze-thaw stability is tested for each product. Most PCR and qPCR products have passed 30 freeze-thaw cycles with no changes in performance. Specific information is found in Storage and Shipping conditions of each product.

When stored and handled under the recommended conditions, full activity of the reagents is retained until the Expiry Date printed on the tube label.

*World Health Organization (2003). Guidelines for the Storage of Essential Medicines and Other Health Commodities.

Storage & Shipping
Storage

Routine storage at -20 °C until expiry date

The mix can be stored at +4 °C for up to 6 months

Stability at room temperature (15 − 25 °C) for 1 month

Freeze-thaw stability: 30 cycles

Shipping

At room temperature