Properties
Concentration: 5x
Hot-start: yes, 3 minutes initial incubation at 95 °C
Cycling speed: fast or ultra-fast
Detection type: Probe-based
Reference dye: none
Multiplex: up to 5 plexes simultaneously
Compatible real-time instruments: The mix is compatible with qPCR cyclers that do not require an internal reference dye (e.g. ROX) for normalization of fluorescent signal. Check Your cycler!
Mix Components:
SolisFAST® DNA Polymerase: In silico- designed analogue of Taq DNA polymerase with enhanced stability at room temperature, fast hot-start and faster extension rates compared to the wild-type Taq DNA polymerase.
qPCR buffer: Includes 15 mM MgCl2 (1x PCR solution 3 mM MgCl2), dNTPs (dATP, dCTP, dGTP, dTTP), additives that maximize efficiency of PCR