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Some applications of this product  may require a license which is not provided by the purchase of this product.

For research use only. 

Highly sensitive dye-based one-step RT-qPCR

SOLIScript® 1-step SolisGreen® Kit is an optimized solution for highly
sensitive real-time one-step RT-PCR (RT-qPCR) using intercalating
dye detection via the SYBR®/FAM fluorescence channel.

  • cDNA synthesis and dye-based qPCR in one tube
  • high specificity, sensitivity and yield
  • reaction set-up and shippment without ice
  • consistent results with low RNA input
  • RNase inhibitor included in kit
  • compatible with most cyclers

Ordering

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Catalog number

08-63-0000S

Quantity

€ 0.00

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Details

Reagents Provided
Item Pcs. Vial size
40x One-step SOLIScript® Mix 1 50 rxn | 25 µl
5x One-step SolisGreen® Mix 1 50 rxn | 200 µl
Water, nuclease free 1 1.25 ml
Overview

Description

SOLIScript® 1-step SolisGreen® Kit is optimized for highly sensitive and accurate quantification of RNA targets by real-time one-step RT-PCR (RT-qPCR) using dsDNA-intercalating dye detection via the SYBR®/FAM fluorescence channel.

Compatible with ROX-independent and ROX-dependent qPCR cyclers (excluding high-ROX platforms).

The Kit comes in 3 tubes (including water) and contains all the components necessary (except RNA template and primers) to perform cDNA synthesis and qPCR in a single tube.

Applications

  • Gene expression analysis and absolute quantification
  • Pathogen detection and quantification
  • Low-copy gene detection
  • Gene knockdown validation
  • miRNA profiling and quantification
  • GMO characterization

Properties

Sample type: RNA

Product format: Reagent kit 

No of tubes: three tubes

Detection type: Intercalating dye-based

Reference dye: reference dye based on ROX

Compatible qPCR cyclers: The Kit is compatible with ROX-independent and ROX-dependent qPCR platforms (excluding high-ROX machines, such as Applied Biosystems™ 5700, 7000, 7700, 7900HT, StepOne™, StepOnePlus™).  Check Your cycler!

Kit Components

40x One-step SOLIScript® Mix: SOLIScript® Reverse Transcriptase, RiboGrip™ RNase Inhibitor

5x One-step SolisGreen® Mix: HOT FIREPol® DNA polymerase, dNTPs (dATP, dCTP, dGTP, dTTP), 12.5 mM MgCl2 (1x RT-qPCR solution – 2.5 mM MgCl2), SolisGreen® dsDNA-intercalating dye, passive reference dye based on ROX dye

Accurate quantification across a broad dynamic range
Sensitive amplification and bright fluorescent signal
The lowest Ct values in competition with other vendors
Related Resources

FAQ

Does RiboGrip help with inhibitor resistance?

RiboGrip™ is not directly associated with tolerance to common inhibitors, however, it helps to keep RNA intact because RiboGrip inhibits Rnase H.

I have previously used SYBR® Green / EvaGreen® based qPCR assays. What are the spectral features of SolisGreen™ dye?

SolisGreen™ nucleic acid dye shares spectral properties with other commonly used qPCR dyes, allowing detection in the FAM or SYBR® Green I channel (SolisGreen™ excitation maximum 489 nm, emission maximum 515 nm). SolisGreen™ is characterized by high sensitivity and great PCR efficiency with low template amounts for accurate and reproducible results. 

SolisGreen™ is based on CYGREEN dye. CYGREEN is used under licence from Enzo Life Sciences, Inc. CYGREEN is a U.S. registered trademark of Enzo Life Sciences, Inc. U.S. Patent Nos. 8,153,802 and 7,569,695.

What is the difference between EvaGreen® and SolisGreen® dye?

Both dyes are DNA intercalating agents that are used to stain dsDNA. SolisGreen™ dye is spectrally similar to EvaGreen® allowing detection in the FAM or SYBR® Green I channel (SolisGreen™ excitation maximum 489 nm, emission maximum 515 nm). It has been shown that qPCR mixes based on SolisGreen™ dye have increased performance with low target concentration demonstrating improved precision and less variance between technical replicates.

Why do certain kits contain a ROX?

ROX (carboxy-X-rhodamine) is one of the passive reference dyes providing a constant fluorescent signal that is used for signal normalization during the amplification cycles. The emission recorded from a reference dye during the baseline cycles is used to normalize the emission recorded from the reporter (e.g. EvaGreen®, SolisGreen®, SYBR® Green, etc.) in later cycles in ROX-dependent real-time PCR systems (e.g. Applied Biosystems 5700, 7000, 7300, 7700, 7900HT, StepOne™, StepOnePlus™). Reference dye compensates for small fluorescent fluctuations and well-to-well variations that may occur.

In Solis BioDyne qPCR mixes we use technology, based on ROX, that allows us to use same mix for high- and low- ROX requiering cyclers. 

Please check cycler-mix compatibility here if not sure.

Storage & Shipping
Storage

Routine storage: -20 ºC

Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of the reagents.

Shipping

At room temperature