Some applications of this product  may require a license which is not provided by the purchase of this product.

For research use only. 

Hot-start DNA Polymerase for MALDI-TOF and other applications with unconventional nucleotides

Thermostable hot-start DNA polymerase for highly efficient incorporation of unconventional nucleotides.

  • efficient incorporation of ddNTPs, acyNTPs, or fluorescent nucleotides
  • the assay success rate of 99% in MALDI-TOF
  • robust and reliable
  • reaction set-up and shipment without dry ice

NB! The name and catalogue number for this product have changed. The components and composition of the product are unchanged. The product formerly named HOT TERMIPol® DNA Polymerase changed to HOT TERMIPol® DNA Polymerase Kit. Former catalogue numbers 01-06-0000S, 01-06-00500, 01-06-02000 changed to 01-06-KIT-0000S, 01-06-KIT-00500, 01-06-KIT-02000 respectively.


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Reagents Provided
Item Pcs. Vial size
HOT TERMIPol® DNA Polymerase 1 500 U | 100 µl
10x Reaction Buffer C 1 5 ml
100 mM MgCl2 1 5 ml


HOT TERMIPol® DNA polymerase is a chemically modified TERMIPol® DNA polymerase. At ambient temperature it is inactive, having no polymerase activity.

HOT TERMIPol® DNA polymerase is fully activated by a 12-15 minute incubation at 95ºC. This prevents the extension of non-specifically annealed primers. The enzyme contains 5’→3’ polymerase activity and enhanced efficiency for incorporating unconventional nucleotides (ddNTPs and labeled ddNTPs).


  • MALDI-TOF mass spectrometry
  • Primer extension
  • Mass-array


Concentration: 5 U/µl

Hot-start: yes, activated by 12-15 minutes at 95ºC

Error rate per nucleotide per cycle is app. 8 x 10-5

Estimated half life at 95ºC is 1.5 hours.

Storage and Dilution Buffer: 50% glycerol (v/v), 20 mM Tris-HCl pH 8.7 at 25ºC, 100 mM KCl, 0.1 mM EDTA and stabilizers.

Source: Purified from an E.coli strain that carries an overproducing plasmid containing a modified gene of Thermus aquaticus DNA Polymerase.

Unit definition: One unit is defined as the amount of enzyme required to catalyze the incorporation of 10 nmol of dNTP into an acid-insoluble form in 30 minutes at 74ºC.


HOT TERMIPol® DNA Polymerase

10x Reaction buffer C: 500 mM Tris-HCl pH 9.5 at 25ºC

100 mM MgCl2

Related Resources


Enzymes are usually very viscous liquids. What would you recommend for the most accurate pipetting?

Pipetting viscous liquids like glycerol with an air displacement micropipette is difficult, because viscous liquids have a high resistance to flow. We have a few recommendations for accurate pipetting:

  • Hold the pipette vertical, since more liquid gets picked up into the tip when the pipette is held at an angle.
  • Only put the pipette tip in the liquid.
  • Use a very slow aspiration speed, wait for a few seconds until the liquid retightens and then use a very slow dispensing speed.
What are the storage conditions and stability of Solis BioDyne reagents?

Solis BioDyne products should be stored at -20°C.

Shipping and temporary storage for up to 1 month at room temperature (*1525°C) has no detrimental effects on the quality of Solis BioDyne reagents.

Freeze-thaw stability is tested for each product. Most PCR and qPCR products have passed 30 freeze-thaw cycles with no changes in performance. Specific information is found in Storage and Shipping conditions of each product.

When stored and handled under the recommended conditions, full activity of the reagents is retained until the Expiry Date printed on the tube label.

*World Health Organization (2003). Guidelines for the Storage of Essential Medicines and Other Health Commodities.

Storage & Shipping

Routine storage: -20ºC
Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of HOT TERMIPol® DNA polymerase.


At room temperature