Some applications of this product may require a license which is not provided by the purchase of this product.
For research use only.
Genetically modified MMLV-based reverse transcriptase with increasedthermostability and improved performance at elevated temperatures
FIREScript® Reverse Transcriptase (RT) is a genetically engineered MMLV (Moloney Murine Leukemia Virus) based Reverse Transcriptase.
This is an RNA‐directed DNA polymerase that can synthesize a complementary DNA strand from ssRNA or ssDNA and is active over a broad range of reaction temperatures from 37°C-60°C.
FIREScript® RT is a robust enzyme for RNA detection and has enhanced stability at room temperature with no activity loss for up to 1 month. This RT contains a functional RNase H domain which can increase the sensitivity of RT‐qPCR (quantitative reverse transcription PCR).
Sample type: RNA
Final product: cDNA (first strand)
Product format: Enzyme and reaction buffer (separate tubes)
Enzyme: FIREScript® Reverse Transcriptase
Source: Purified from an E.coli strain that carries an overproducing plasmid containing a FIREScript® Reverse Transcriptase gene.
Unit definition: One unit is defined as the amount of enzyme that will incorporate 1 nmol of dTTP into acid-precipitable material in 10 minutes at 37°C using poly(A)•oligo(dT) as a template in a total reaction volume of 50 μl.
Storage and dilution buffer: 50% glycerol (v/v), 20 mM Tris-HCl pH 7.5 at 25°C, 100 mM KCl, 0.1 mM EDTA and stabilizers.
FIREScript® Reverse Transcriptase (200 U/μl)
10x RT Reaction Buffer with DTT (500 mM Tris-HCl pH 8.3, 500 mM KCl, 30 mM MgCl2, 100 mM DTT)
Pipetting viscous liquids like glycerol with an air displacement micropipette is difficult, because viscous liquids have a high resistance to flow. We have a few recommendations for accurate pipetting:
Solis BioDyne products should be stored at -20°C.
Shipping and temporary storage for up to 1 month at room temperature (*15−25°C) has no detrimental effects on the quality of Solis BioDyne reagents.
Freeze-thaw stability is tested for each product. Most PCR and qPCR products have passed 30 freeze-thaw cycles with no changes in performance. Specific information is found in Storage and Shipping conditions of each product.
When stored and handled under the recommended conditions, full activity of the reagents is retained until the Expiry Date printed on the tube label.
*World Health Organization (2003). Guidelines for the Storage of Essential Medicines and Other Health Commodities.
Routine storage: -20ºC
Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of the reagents.
At room temperature
A flexible and robust cDNA synthesis kit contains all the reagents you need for first-strand cDNA synthesis. Individual components enable thorough assay optimization.
All-inclusive and the most convenient product format for first-strand cDNA synthesis. A combined enzyme mix (FIREScript® and RiboGrip®) and a reaction premix in separate tubes allowing you to choose the most appropriate priming option.
