Properties
Sample type: RNA
Final product: cDNA (first strand)
Product format: Enzyme and reaction buffer (separate tubes)
Enzyme: FIREScript® Reverse Transcriptase
Source: Purified from an E.coli strain that carries an overproducing plasmid containing a FIREScript® Reverse Transcriptase gene.
Unit definition: One unit is defined as the amount of enzyme that will incorporate 1 nmol of dTTP into acid-precipitable material in 10 minutes at 37°C using poly(A)•oligo(dT) as a template in a total reaction volume of 50 μl.
Storage and dilution buffer: 50% glycerol (v/v), 20 mM Tris-HCl pH 7.5 at 25°C, 100 mM KCl, 0.1 mM EDTA and stabilizers.
Reagents
FIREScript® Reverse Transcriptase (200 U/μl)
10x RT Reaction Buffer with DTT (500 mM Tris-HCl pH 8.3, 500 mM KCl, 30 mM MgCl2, 100 mM DTT)