FIREPol^® Master Mix

The concentration of the MgCl₂ in PCR reaction affects the productivity of your PCR reaction. Higher concentrations of MgCl₂ may increase productivity of DNA polymerase, but also may decrease specificity causing unexpected products.

Usually, 1.5-2.5 mM final concentration of MgCl₂ is optimal for general PCR. However, optimal concentration of MgCl₂ depends on template, buffer, DNA and dNTPs. Solis BioDyne produces Master Mixes at different MgCl₂ concentration, which allows greater flexibility in reaction optimisation. For your very first experiment with Master Mix, we recommend to use Master Mix with 1,5 - 2 mM MgCl₂ (in final 1x PCR Mix)

Solis BioDyne products should be stored at -20°C.

Shipping and temporary storage for up to 1 month at room temperature (*15−25°C) has no detrimental effects on the quality of Solis BioDyne reagents.

Freeze-thaw stability is tested for each product. Most PCR and qPCR products have passed 30 freeze-thaw cycles with no changes in performance. Specific information is found in Storage and Shipping conditions of each product.

When stored and handled under the recommended conditions, full activity of the reagents is retained until the Expiry Date printed on the tube label.

*World Health Organization (2003). Guidelines for the Storage of Essential Medicines and Other Health Commodities.