Some applications of this product may require a license which is not provided by the purchase of this product.
For research use only.
Optimized ready-to-use PCR master mix for routine PCR assays. This master mix contains thermostable Taq polymerase FIREPol®, MgCl2, dNTPs, and buffer with detergent. You just need to add a template, primers, and water.
We recommend using FIREPol® Master Mix without ready to load feature in all PCR applications where spectrophotometric measurements (absorbance or fluorescence) are necessary post-PCR because loading dyes can interfere with these applications.
Concentration: 5x
Hot-start: no
Ready to load: no
Fidelity: same as Taq
Cloning Type: T/A Cloning
Amplicon Size: up to 5 kb
FIREPol® DNA polymerase: highly processive. thermostable DNA polymerase
5x Reaction Buffer B: 0.4 M Tris-HCl, 0.1 M (NH4)2SO4, 0.1% w/v Tween-20
7.5 mM MgCl2: 1x PCR solution – 1.5 mM MgCl2)
1 mM dNTPs of each: 1x PCR solution – 200 µM dATP, 200 µM dCTP, 200 µM dGTP and 200 µM dTTP
The concentration of the MgCl2 in PCR reaction affects the productivity of your PCR reaction. Higher concentrations of MgCl2 may increase productivity of DNA polymerase, but also may decrease specificity causing unexpected products.
Usually, 1.5-2.5 mM final concentration of MgCl2 is optimal for general PCR. However, optimal concentration of MgCl2 depends on template, buffer, DNA and dNTPs. Solis BioDyne produces Master Mixes at different MgCl2 concentration, which allows greater flexibility in reaction optimisation. For your very first experiment with Master Mix, we recommend to use Master Mix with 1,5 - 2 mM MgCl2 (in final 1x PCR Mix)
Solis BioDyne products should be stored at -20°C.
Shipping and temporary storage for up to 1 month at room temperature (*15−25°C) has no detrimental effects on the quality of Solis BioDyne reagents.
Freeze-thaw stability is tested for each product. Most PCR and qPCR products have passed 30 freeze-thaw cycles with no changes in performance. Specific information is found in Storage and Shipping conditions of each product.
When stored and handled under the recommended conditions, full activity of the reagents is retained until the Expiry Date printed on the tube label.
*World Health Organization (2003). Guidelines for the Storage of Essential Medicines and Other Health Commodities.
Routine storage: -20ºC
Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of FIREPol® Master Mix
At room temperature
FIREPol® is a highly processive, thermostable Taq DNA polymerase with unique 30-day stability at room temperature.
Convenient Master Mix for standard PCR applications, based on FIREPol® DNA Polymerase. Includes two tracking dyes for direct loading on gel.
Robust and reliable hot-start Master Mix with higher fidelity and longer amplification range for more demanding reactions.
Convenient ready-to-load molecular weight marker to determine the size of dsDNA during gel electrophoresis. Contains 13 discrete bands ranging from 250 bp to 10 000 bp.
Convenient ready-to-load molecular weight marker to determine the size of dsDNA during gel electrophoresis. Contains 13 discrete bands ranging from 100 bp to 3000 bp.
For loading and tracking DNA samples on gel. Visible blue dyes comigrate with 30 bp and 3500 bp fragments (in 1x TBE) or 60 bp and 5000 bp fragments (in 1x TAE) on 1% agarose gel.
