6x DNA Loading Dye Buffer Blue

The 6X Loading Dye buffer is used to prepare DNA ladders and samples for loading on agarose or polyacrylamide gels.

Loading Dye Buffers contain glycerol that ensures that the DNA in the ladder and sample forms a layer at the bottom of the well, and color markers (e.g. Bromophenol blue, Xylene cyanole FF, Orange G) for visual tracking of DNA migration during electrophoresis.

The concentration of agarose in a gel depends on the sizes of the DNA fragments, and should be able to separate your fragment(s) enough so that they are identifiable.

Smaller fragments of DNA migrate through the gel more quickly and, therefore, are separated on higher concentrations of agarose. Larger molecules require a lower concentration of agarose.

Dilute your DNA sample with the 6x DNA Loading Dye Buffer: mix 1 volume of the Loading Dye Buffer with 5 volumes of your DNA sample.

Loading Dye Buffers contain glycerol that ensures that the DNA in the ladder and sample forms a layer at the bottom of the well, and color markers (e.g. Bromophenol blue, Xylene cyanole FF, Orange G) for visual tracking of DNA migration during electrophoresis.

For the best results, use Solis BioDyne 6x Loading Dye Buffers (Blue Dye Cat. No. 07-01-00010, Double Blue Dye 07-02-00010, Orange Dye 07-04-00010, Orange Blue Dye 07-03-00010).