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For research use only. 

Stock solution for PCR assay optimization

25 mM MgCl2 is used for adjusting Mg2+ concentration in PCR assay.

  • additive for (q)PCR
  • additive for RT-(q)PCR


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MgCl2 solution is used for adjusting Mg2+ concentration in PCR assay. It can be used in combination with PCR buffers that come with FIREPol® and HOT FIREPol® which are all MgCl2 free). In most applications, a final concentration of 1,5 mM MgCl2 is optimal for a satisfactory yield. In some cases, MgCl2 titration is needed for a better yield and specificity.

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Routine storage: -20ºC
Temporary storage at room temperature has no detrimental effects on the quality of this reagent.


At room temperature