Probably one of the hottest methods right now in the world of science is PCR (polymerase chain reaction). What started in 1983 as a simple method to amplify DNA now has many different variations and endless application possibilities. As of today, PCR reached the “golden standard” status and is the most applied method in molecular diagnostics with hard-to-beat accuracy and reliability. PCR is routinely applied in infectious disease diagnostics, but also genetic, oncology, prenatal screenings. I doubt that the PCR inventor would have ever guessed that this would become the method eventually transferring the whole healthcare industry. Today, using the same PCR reaction we are basically able to detect anything within minutes, which is a powerful and irreplaceable tool in the hands of medical professionals.
The most popular variations are probably the conventional PCR aka endpoint PCR and qPCR also known as real-time PCR or RT-PCR. To avoid any confusion we are going with endpoint PCR and qPCR.
Both of these variations have a quite similar general mechanism. First, you need purified DNA (not mandatory, but recommended). The larger part of the PCR reaction occurs in three main steps, repeated around 20-40 times.
Every company has to start from somewhere. There can either be an “aha” moment - a sudden spark of inspiration that sets everything in motion. Other times it can happen gradually with ideas coming and going until something bigger grows out of all this brainstorming. For Solis BioDyne, it was the latter. As we celebrate 30 years since our founding, we think it’s the perfect time to share our origin story with you.
Have you ever thought about doing a PCR experiment, but you are not quite sure what product to use? Or the product you have chosen doesn’t work as well as it should and you are looking for an alternative? We are here to help you out with some recommendations from fellow scientists based on different application possibilities.
For 10 years now, on the 11th of February, we celebrate the women and girls who have chosen to follow the path of science. We believe giving everyone a chance to do what they love is important, and it doesn’t matter what gender a person is. In Solis BioDyne, 66% of our employees and 75% of our scientists are women and they are absolutely amazing in their work.
To advance innovation in synthetic biology we decided to help young and talented scientists from Lund University with their Methane RemOOver project. Their goal is the reduction of methane emissions from cows using a synthetically engineered microorganism. With this idea, they also participated in iGEM competition.