Remarkable stability at higher temperatures. Amplification plot showcasing results of stability testing with HOT FIREPol® SolisGreen® qPCR Mix 2.0. Stability testing was carried out with TUBA8 target on four 10-fold dilutions (20 ng to 20 pg) of human gDNA using Quantstudio™ 6 Pro qPCR cycler (Applied Biosystems™). The results demonstrate great sensitivity and reproducibility with high fluorescence levels when tested for 2 weeks at 37°C (yellow), and 1 month at room temperature (25 °C, red) in comparison of a product kept at -20 °C as a reference (blue).
Accurate quantification across a broad dynamic range! One-step RT-qPCR amplification plot showcasing extreme accuracy across a broad dynamic range. SOLIScript® 1-step SolisGreen® Kit 2.0 was used to target PPIA gene fragment using eight 10-fold serial dilutions (0.1 pg–1 μg, six replicates at each concentration) of Human Reference total RNA. Reactions were run on QuantStudio™ 6 Flex qPCR cycler (Applied BioSystems™).
HOT FIREPol® SolisGreen® qPCR Mix 2.0 is for qPCR (for analyzing DNA samples). In addition to other applications, it allows you to do cell-free analysis.
SOLIScript® 1-step SolisGreen® Kit 2.0 is for RT-qPCR (for analyzing RNA samples).
Dye-based qPCR is very cost-effective when planning a large-scale experiment. It saves time during the experimental design phase because it only requires the proper design of primers. It’s easy to use - straightforward, with fewer reagents needed compared to probe-based methods. While in terms of specificity dye-based qPCR is not as good as probe-based options, it’s highly sensitive and capable of detecting low amounts of DNA due to the strong fluorescence signal upon binding to dsDNA. Dye-based qPCR also allows you to detect non-specific binding, which probe-based qPCR can’t do. This is especially useful during the starting phase of experiments to make sure that you are seeing correct results.
However, if you are looking to multiplex or want results with very high specificity, probe-based qPCR may be more suitable for you. Luckily, we also provide solutions for that.
HOT FIREPol® SolisGreen® qPCR Mix 2.0
SOLIScript® 1-step SolisGreen® Kit 2.0
Let’s talk about epigenetics! In this post, we’ll break down how the gold standard method for studying DNA methylation - bisulfite sequencing - works, why it matters in fields like cancer research and developmental biology, and what makes it such a cornerstone in the study of the epigenome.
For many years now, the Genetics Workshops have brought hands-on science to Estonian high school students, offering a rare and valuable opportunity: the chance to conduct real genetic experiments in a university lab — and a glimpse into the fascinating world of genetics.
We are proud to present Solis Hot Start HiFi DNA Polymerase, a high-performance, high-fidelity enzyme designed for precision-demanding PCR applications such as sequencing, cloning, and mutagenesis. This product has superior performance with a variety of amplicons, can be used for long-range and challenging PCR, and has broad multiplexing capabilities.
It doesn’t happen often that products developed almost three decades ago remain not only on the product list but as one of the most popular products overall. This is, however, the case with our main polymerases. These have become classics that seem to never go out of style like jeans or a little black dress. So, what makes them so special? Let’s take a look!
