Remarkable stability at higher temperatures. Amplification plot showcasing results of stability testing with HOT FIREPol® SolisGreen® qPCR Mix 2.0. Stability testing was carried out with TUBA8 target on four 10-fold dilutions (20 ng to 20 pg) of human gDNA using Quantstudio™ 6 Pro qPCR cycler (Applied Biosystems™). The results demonstrate great sensitivity and reproducibility with high fluorescence levels when tested for 2 weeks at 37°C (yellow), and 1 month at room temperature (25 °C, red) in comparison of a product kept at -20 °C as a reference (blue).
Accurate quantification across a broad dynamic range! One-step RT-qPCR amplification plot showcasing extreme accuracy across a broad dynamic range. SOLIScript® 1-step SolisGreen® Kit 2.0 was used to target PPIA gene fragment using eight 10-fold serial dilutions (0.1 pg–1 μg, six replicates at each concentration) of Human Reference total RNA. Reactions were run on QuantStudio™ 6 Flex qPCR cycler (Applied BioSystems™).
HOT FIREPol® SolisGreen® qPCR Mix 2.0 is for qPCR (for analyzing DNA samples). In addition to other applications, it allows you to do cell-free analysis.
SOLIScript® 1-step SolisGreen® Kit 2.0 is for RT-qPCR (for analyzing RNA samples).
Dye-based qPCR is very cost-effective when planning a large-scale experiment. It saves time during the experimental design phase because it only requires the proper design of primers. It’s easy to use - straightforward, with fewer reagents needed compared to probe-based methods. While in terms of specificity dye-based qPCR is not as good as probe-based options, it’s highly sensitive and capable of detecting low amounts of DNA due to the strong fluorescence signal upon binding to dsDNA. Dye-based qPCR also allows you to detect non-specific binding, which probe-based qPCR can’t do. This is especially useful during the starting phase of experiments to make sure that you are seeing correct results.
However, if you are looking to multiplex or want results with very high specificity, probe-based qPCR may be more suitable for you. Luckily, we also provide solutions for that.
HOT FIREPol® SolisGreen® qPCR Mix 2.0
SOLIScript® 1-step SolisGreen® Kit 2.0
On the 4th and 5th of June representatives from South Korea visited Tartu. Their primary focus was on exploring opportunities in the fields of biotechnology and medicine, seeking potential collaborators in Estonia. We had the great pleasure of meeting the delegation and sharing our knowledge on both days of the visit.
Lyophilization, also known as freeze-drying, is in simple terms a water-removal process that increases product stability and preserves its functionality. Our new SolisFAST® Lyo-Ready qPCR Kit with UNG represents an optimized lyophilization-compatible qPCR solution to enhance the simplicity, convenience, and speed of diagnostic and applied testing.
The running joke with PCR is that if something can go wrong, it will go wrong. Quite often it’s even impossible to determine why some samples turned out fine while the others did not. In a situation like this, it would be amazing to know some trick or a secret to avoid spending all the time and resources to do the experiment again. Here are a few we are willing to share so that you could find love for PCR.
In research, every day different methods are used to discover something new, whether it is a new disease, medicine, or something else. Often these methods were developed long ago and are confirmed to be doing what they are supposed to do. However, as technology develops so do new methods. This is exactly what Professor Steven Williams’ lab is doing at Smith College – developing new methods to be used in research and diagnostics.
