Remarkable stability at higher temperatures. Amplification plot showcasing results of stability testing with HOT FIREPol® SolisGreen® qPCR Mix 2.0. Stability testing was carried out with TUBA8 target on four 10-fold dilutions (20 ng to 20 pg) of human gDNA using Quantstudio™ 6 Pro qPCR cycler (Applied Biosystems™). The results demonstrate great sensitivity and reproducibility with high fluorescence levels when tested for 2 weeks at 37°C (yellow), and 1 month at room temperature (25 °C, red) in comparison of a product kept at -20 °C as a reference (blue).
Accurate quantification across a broad dynamic range! One-step RT-qPCR amplification plot showcasing extreme accuracy across a broad dynamic range. SOLIScript® 1-step SolisGreen® Kit 2.0 was used to target PPIA gene fragment using eight 10-fold serial dilutions (0.1 pg–1 μg, six replicates at each concentration) of Human Reference total RNA. Reactions were run on QuantStudio™ 6 Flex qPCR cycler (Applied BioSystems™).
HOT FIREPol® SolisGreen® qPCR Mix 2.0 is for qPCR (for analyzing DNA samples). In addition to other applications, it allows you to do cell-free analysis.
SOLIScript® 1-step SolisGreen® Kit 2.0 is for RT-qPCR (for analyzing RNA samples).
Dye-based qPCR is very cost-effective when planning a large-scale experiment. It saves time during the experimental design phase because it only requires the proper design of primers. It’s easy to use - straightforward, with fewer reagents needed compared to probe-based methods. While in terms of specificity dye-based qPCR is not as good as probe-based options, it’s highly sensitive and capable of detecting low amounts of DNA due to the strong fluorescence signal upon binding to dsDNA. Dye-based qPCR also allows you to detect non-specific binding, which probe-based qPCR can’t do. This is especially useful during the starting phase of experiments to make sure that you are seeing correct results.
However, if you are looking to multiplex or want results with very high specificity, probe-based qPCR may be more suitable for you. Luckily, we also provide solutions for that.
HOT FIREPol® SolisGreen® qPCR Mix 2.0
SOLIScript® 1-step SolisGreen® Kit 2.0
Valentine’s Day is a celebration of connection, commitment, love, and the dream of starting a family. However, sometimes human relationships need a little help from advances in science. In vitro fertilization (IVF), one of the most important assisted reproductive technologies, has helped millions of people start families. Yet behind every successful embryo transfer lies a deeper, invisible layer of biology - molecular precision.
It is always inspiring to see what young scientists can achieve when given the opportunity to tackle real-world challenges. This year, we supported a student team Trashformers from Lund University as they were participating in the iGEM competition. Their project’s goal was to tackle the critical issue of textile waste, specifically focusing on recycling polyester.
Have you ever sat around a table with your friends or family and just come up with the wildest ideas while having a good meal? One of Kadri’s brightest memories is exactly that — sitting around a table during lunch with her husband, sister, and brother-in-law Olev, and having the conversation that led to the start of Solis BioDyne.
Our last story ended in the middle of 2005. By then, we had spent a decade developing room-temperature stable DNA polymerases for a range of applications. In the second half of that year, we reached a turning point – we started blending and mixing to help scientists around the world reduce pipetting errors and save time.
