Remarkable stability at higher temperatures. Amplification plot showcasing results of stability testing with HOT FIREPol® SolisGreen® qPCR Mix 2.0. Stability testing was carried out with TUBA8 target on four 10-fold dilutions (20 ng to 20 pg) of human gDNA using Quantstudio™ 6 Pro qPCR cycler (Applied Biosystems™). The results demonstrate great sensitivity and reproducibility with high fluorescence levels when tested for 2 weeks at 37°C (yellow), and 1 month at room temperature (25 °C, red) in comparison of a product kept at -20 °C as a reference (blue).
Accurate quantification across a broad dynamic range! One-step RT-qPCR amplification plot showcasing extreme accuracy across a broad dynamic range. SOLIScript® 1-step SolisGreen® Kit 2.0 was used to target PPIA gene fragment using eight 10-fold serial dilutions (0.1 pg–1 μg, six replicates at each concentration) of Human Reference total RNA. Reactions were run on QuantStudio™ 6 Flex qPCR cycler (Applied BioSystems™).
HOT FIREPol® SolisGreen® qPCR Mix 2.0 is for qPCR (for analyzing DNA samples). In addition to other applications, it allows you to do cell-free analysis.
SOLIScript® 1-step SolisGreen® Kit 2.0 is for RT-qPCR (for analyzing RNA samples).
Dye-based qPCR is very cost-effective when planning a large-scale experiment. It saves time during the experimental design phase because it only requires the proper design of primers. It’s easy to use - straightforward, with fewer reagents needed compared to probe-based methods. While in terms of specificity dye-based qPCR is not as good as probe-based options, it’s highly sensitive and capable of detecting low amounts of DNA due to the strong fluorescence signal upon binding to dsDNA. Dye-based qPCR also allows you to detect non-specific binding, which probe-based qPCR can’t do. This is especially useful during the starting phase of experiments to make sure that you are seeing correct results.
However, if you are looking to multiplex or want results with very high specificity, probe-based qPCR may be more suitable for you. Luckily, we also provide solutions for that.
HOT FIREPol® SolisGreen® qPCR Mix 2.0
SOLIScript® 1-step SolisGreen® Kit 2.0
Every company has to start from somewhere. There can either be an “aha” moment - a sudden spark of inspiration that sets everything in motion. Other times it can happen gradually with ideas coming and going until something bigger grows out of all this brainstorming. For Solis BioDyne, it was the latter. As we celebrate 30 years since our founding, we think it’s the perfect time to share our origin story with you.
Have you ever thought about doing a PCR experiment, but you are not quite sure what product to use? Or the product you have chosen doesn’t work as well as it should and you are looking for an alternative? We are here to help you out with some recommendations from fellow scientists based on different application possibilities.
For 10 years now, on the 11th of February, we celebrate the women and girls who have chosen to follow the path of science. We believe giving everyone a chance to do what they love is important, and it doesn’t matter what gender a person is. In Solis BioDyne, 66% of our employees and 75% of our scientists are women and they are absolutely amazing in their work.
To advance innovation in synthetic biology we decided to help young and talented scientists from Lund University with their Methane RemOOver project. Their goal is the reduction of methane emissions from cows using a synthetically engineered microorganism. With this idea, they also participated in iGEM competition.
