The scientific community is using multiplexing on a daily basis to gain valuable insight into the world's most crucial issues. In diagnostic tests requiring multiple different targets to be detected, multiplexing offers a way to increase efficiency. Often, a quick and specific differential diagnosis is needed to confirm or rule out a number of potential pathogens responsible for a specific infection. Combined with reverse transcription, multiplex PCR and qPCR are also applicable in detecting several viral pathogens with RNA as genetic material. An example would be upper respiratory infections, where a number of viruses (eg. rhinovirus, influenza virus, human coronavirus) can cause similar and non-specific symptoms.
Malaria is a disease that is relevant in many parts of the world. Multiplex qPCR is a method that has shown its superior performance in achieving high-throughput screening of the disease. Additionally, its reduced cost makes it a desirable choice for epidemiological and surveillance studies.
A fast differentiation between bacterial and viral pathogens is also needed for an accurate treatment plan and improved patient outcomes for patients with pneumonia. Smaller organ groups, such as eyes or joints, also suffer from infections of different origins and a correct diagnosis is crucial for applying the correct therapy. To illustrate, Chlamydia trachomatis, herpes simplex virus (HSV), and adenoviruses are the common causes of keratoconjunctivitis and a multiplex PCR reaction enables differentiation between them from just one conjunctival swab sample. The same principle applies to many other sample types, such as saliva, blood, and urine samples and their most common pathogens.
Many veterinary laboratories also benefit from multiplex PCR tests, as different bacterial, viral, fungal, and parasitic infections are no strangers to the zoological world. In the veterinary sector, diagnostic laboratories are often geographically far from the barns and stables, so samples often need to be shipped to testing facilities. Therefore, taking additional samples later may not be an option. Again, multiplex PCR enables the detection of various pathogens and markers from the same sample to get to the right diagnosis as fast as possible.
Multiplex PCR can be useful in many different fields. When working with plants, cell cultures, laboratory animals, microorganisms, diagnostics, or forensics, consider your options to make your road to data faster and more effective. Here at Solis BioDyne, we feel honored to be able to do our part and have our products as the tools of choice for key areas such as cancer research among others.
Solis BioDyne multiplex-ready probe-based qPCR Master Mixes:
For fast cycling speed and up to 5-plex reactions:
For standard cycling speed and up to 4-plex reactions:
By using the right product designed for multiplexing, you can rest assured you will enjoy similar sensitivity to singleplexing with a greatly reduced workload.
We are proud to present Solis Hot Start HiFi DNA Polymerase, a high-performance, high-fidelity enzyme designed for precision-demanding PCR applications such as sequencing, cloning, and mutagenesis. This product has superior performance with a variety of amplicons, can be used for long-range and challenging PCR, and has broad multiplexing capabilities.
It doesn’t happen often that products developed almost three decades ago remain not only on the product list but as one of the most popular products overall. This is, however, the case with our main polymerases. These have become classics that seem to never go out of style like jeans or a little black dress. So, what makes them so special? Let’s take a look!
Every company has to start from somewhere. There can either be an “aha” moment - a sudden spark of inspiration that sets everything in motion. Other times it can happen gradually with ideas coming and going until something bigger grows out of all this brainstorming. For Solis BioDyne, it was the latter. As we celebrate 30 years since our founding, we think it’s the perfect time to share our origin story with you.
Have you ever thought about doing a PCR experiment, but you are not quite sure what product to use? Or the product you have chosen doesn’t work as well as it should and you are looking for an alternative? We are here to help you out with some recommendations from fellow scientists based on different application possibilities.
