5th December is World Soil Day!

The Food and Agriculture Organization of the United Nations is celebrating World Soil Day!

This year’s campaign is called „Keep soil alive, protect soil biodiversity “. It aims to raise awareness of the importance of sustaining healthy ecosystems and human well-being.

We are very happy that many scientists from all over the world have decided to use Solis BioDyne’s products to contribute to soil research. Here are a few summaries of publications showing the usage of our products in that field.

  • Kępczyńska, E. & Karczyński, P. Medicago truncatula root developmental changes by growth-promoting microbes isolated from Fabaceae, growing on organic farms, involve cell cycle changes and WOX5 gene expression. Planta (2020)

Soil is home to many different bacteria. One group of them, called plant growth-promoting bacteria (PGPB), offers an environmentally safe alternative to synthetic fertilizers and pesticides used to improve the growth and health of plants.

A group of scientists from Poland (Kępczyńska, E. & Karczyński, P) conducted a screening of seventy-two bacterial isolates from rhizospheric soil and root nodule samples of legumes for their growth-promoting capacities. They saw that the inoculation of seedlings with plant growth-promoting bacteria resulted in enhanced root and shoot weight and a more branched root system. Using Solis BioDyne’s HOT FIREPol® EvaGreen® qPCR Mix Plus in their qPCR experiments, they also discovered that three selected bacterial strains were able to enhance the expression of the WOX5 gene in roots, which is associated with promoting the growth of root tips and lateral zones.

Great to see such discoveries about environmentally friendly solutions in agriculture!

  • Loit, K. et al. Temporal and Cultivar-Specific Effects on Potato Root and Soil Fungal Diversity. Agronomy (2020)

Potato is a popular crop all around the world. However, potato plants are exposed to various pathogens. A group of Estonian scientists (Loit et al.) decided to take a deeper look into soil-borne fungal pathogens and genotypic differences in biotic stress resistance in potato plants.

They extracted DNA from the roots and soil of 21 potato cultivars and identified the fungal and pathogenic species of the samples by amplifying the ITS2 region using Solis BioDyne’s HOT FIREPol® Blend Master Mix. The amplicons were then subjected to high-throughput sequencing. Their results show changes of soil and root fungal communities of potato over the growing season and demonstrate that in roots, the cultivar is the primary factor determining overall fungal, pathogen, and saprotroph community composition.

Understanding the exact fungal pathogens associated with certain cultivars can lead to more environmentally friendly decisions in increasing the yield of the crop and thinking about healthier soil ecosystems!

  • Sun, Z. et al. Contrasting effects of biochar on N2O emission and N uptake at different N fertilizer levels on a temperate sandy loam. Sci. Total Environ. (2017)

It has been shown that global warming is tightly bound to N2O emission from agricultural production due to mineral or organic fertilizers used in soil. Fortunately, biochar has been seen as a promising solution in mitigating that effect. Biochar is a stable solid, rich in carbon that is produced by heating organic material in a zero- or low-oxygen environment.

A group of German scientists contributed to better understand the effect of biochar. Different conditions and compositions of biochar and N fertilizers were studied. In addition to these aspects, they also examined the microbial community composition and the abundance of the functional gene, nosZ, that also plays part in N2O transformation. They extracted DNA from soil samples and measured the abundance of nosZ gene using our HOT FIREPol® EvaGreen® HRM Mix and found that the mean of the nosZ gene did not differ in biochar-treated and untreated samples. However, changes in other aspects led them to conclude that biochar can reduce the greenhouse gas effect at high levels of N supply and may stimulate nutrient uptake when no N is added.

Studying the ways of soil management that can reduce global warming can lead to a better and healthier environment in the future!

Here is an additional selection of publications using our products on soil research:

  •  Cavael, U., Diehl, K. & Lentzsch, P. Assessment of growth suppression in apple production with replant soils. Ecol. Indic. (2020) (HOT FIREPol® Evagreen® HRM Mix)
  •  Pent, M., Bahram, M. & Põldmaa, K. Fruitbody chemistry underlies the structure of endofungal bacterial communities across fungal guilds and phylogenetic groups. ISME J. (2020) (HOT FIREPol® Blend Master Mix)
  •  Delavaux, C. S. et al. Root pathogen diversity and composition varies with climate in undisturbed grasslands, but less so in anthropogenically disturbed grasslands. ISME J. (2020) (HOT FIREPol® DNA Polymerase)
  • Bukovská, P. et al. Utilization of organic nitrogen by arbuscular mycorrhizal fungi---is there a specific role for protists and ammonia oxidizers? Mycorrhiza (2018) (HOT FIREPol® Evagreen® qPCR Mix Plus, HOT FIREPol® Probe qPCR Mix Plus)


Our newest and most robust endpoint PCR mix - SolisFAST® Master Mix with UNG  – has received great feedback from French Guyana for its inhibitor tolerance in difficult soil samples.

I first attempted to amplify an insect DNA gene with HOT FIREPol® Blend Master Mix in soil samples containing many inhibitors. The few amplicons obtained were non-specific. I then tested the Solis FAST Master Mix UNG on the same samples and was finally able to get a better yield and specific amplification of my target gene
Melle Eliane LOUISANNA,
UMR ECOlogie des FOrêts de Guyane (ECOFOG)