Some applications of this product  may require a license which is not provided by the purchase of this product.

For research use only. 

Sensitive and highly specific cDNA synthesis and end-point PCR in a single tube.

  • Faster than ever – under 1-hour RT-PCR cycling protocol
  • Superior sensitivity – detect as little as 0.1 pg of RNA
  • Discover the convenience of multiplexing – amplify up to 5 targets within one reaction
  • Prevent carry-over contamination with preblended UNG enzyme
  • Enhance sensitivity and tackle GC-rich amplicons with AmpliBoost™ RT-PCR enhancer
  • Inherently stable due to proprietary Stability TAG technology
  • Ready to Load version available to streamline your gel electrophoresis workflow


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Reagents Provided
Item Pcs. Vial size
4x One-step HOT SolisFAST® Mix with UNG 1 50 rxn | 50 rxn | 250 μl
40x One-step RT Mix 1 50 rxn | 25 µl
Water, nuclease free 1 1.25 ml
5x AmpliBoost™ RT-PCR Enhancer 1 50 rxn | 50 rxn | 200 μl


SolisFAST® 1-step RT-PCR Kit with UNG provides a simple and streamlined workflow for analyzing RNA targets using reverse transcription PCR (RT-PCR). Both the cDNA synthesis and PCR are conveniently performed in one tube greatly simplifying the analysis workflow, reducing reaction-to-reaction variation, minimizing hands-on steps and reducing reaction set-up to results time.

Exceptionally fast and robust SolisFAST® polymerase combined with our highly processive reverse transcriptases make SolisFAST® 1-step RT-PCR Kit with UNG the fastest kit on the market to perform RT-PCR with unparalleled under 1-hour reaction times. Optimized composition ensures superior sensitivity allowing detection of as little as 0.1 pg of RNA and enabling efficient multiplexing of up to 5 RNA targets simultaneously.

Combination of our highly thermostable FIREScript® and SOLIScript® reverse transcriptases allow efficient reactions at temperatures up to 60 °C to facilitate amplifying difficult RNA targets with challenging secondary structures. The RT mix is preblended with our in silico-designed ribonuclease inhibitor RiboGrip® to minimize the risk of RNA degradation. Salini UNG® Uracil-N-Glycosylase is incorporated into the product along with dUTPs to prevent carryover contamination and false positive results.

The kit is supplied with a novel RT-PCR enhancer – AmpliBoostTM to further improve sensitivity and performance with GC-rich and other challenging amplicons.

Additionally, the kit is room temperature stable for up to 1 month due to the use of Stability TAG - Solis BioDyne’s proprietary and patented polypeptide stabilization technology that is incorporated into each enzyme, enabling environmentally friendly ambient temperature shipping [1].

[1] Kahre, O. et al., Compositions for increasing polypeptide stability and activity, and related methods, EP2501716B1 (2015) and US9321999B2 (2016)


SolisFAST® 1-step RT-PCR Kit with UNG is suitable for RT-PCR applications such as:

  • Gene expression analysis
  • Determining the presence or absence of RNA
  • Virus detection
  • Cloning of RNA templates up to 2 kb
  • Suitable for both singleplex or multiplex (up to 5-targets) RT-PCR reactions


Sample type: RNA

Product format: reagent kit

Detection type: end-point analysis 

Priming method: gene specific primers

Ready-to-load: no

Amplicon Size: up to 2 kb

Targets: 1-5 targets


4x One-step HOT SolisFAST® Mix with UNG with HOT SolisFAST® DNA polymerase, Salini UNG® Uracil-N-Glycosylase, optimized reaction buffer, dNTPs including dUTP, 6.4 mM MgCl2 (1x qPCR solution – 1.6 mM MgCl2)

40x One-step RT Mix with SOLIScript® Reverse Transcriptase, FIREScript® Reverse Transcriptase, RiboGrip® RNase Inhibitor, Salini UNG® Uracil-N-Glycosylase.

5x AmpliBoost™ RT-PCR enhancer – A novel RT-PCR enhancer to improve sensitivity and help with challenging templates (e.g. GC-rich, secondary RNA structures).

Water, nuclease free

Storage & Shipping

Routine storage at -20 °C.

Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of the reagents.


At room temperature.