Some applications of this product may require a license which is not provided by the purchase of this product.
For research use only.
In the new SoliSD™ Lyo-compatible RT-LAMP Kit all enzymes are compatible with freeze- and/or air-drying and contain the patented Stability TAG. The kit is provided in a flexible 5-vial format, with the unique 300X RT-Mix consisting of the blend of Solis BioDyne reverse transcriptases and RNase inhibitor.
NB! This is a special product. To place an order or ask for the price offer contact orders@solisbiodyne.com.
SoliSD™ Glycerol-Free Bsm DNA Polymerase is based on the Large Fragment of the DNA Polymerase from the genus Bacillus smithii with functional similarity to other Bst and Bsm DNA polymerases. Implemented Stability TAG technology [1] ensures incredible stability for at least 1 month at 37°C. The enzyme has a strong strand displacement DNA polymerase activity and lacks 5’→3’ and 3’→5’ exonuclease activities.
SoliSD™ Lyo-compatible RT-LAMP Kit is provided in a 5-vial format, where composition gives flexibility for developing your own isothermal amplification assay.
[1] Kahre, O. et al., Compositions for increasing polypeptide stability and activity, and related methods, EP2501716B1 (2015) and US9321999B2 (2016).
Lyophilization series:
To protect the biological material, such as enzymes, from the temperature and pressure changes during lyophilization, freeze-drying protectants are employed. These protecting excipients ensure that the materials reserve their active state after lyophilisation. In addition to that, they provide an adequate structure to the lyophilized cake or bead.
Lyophilization, commonly known as freeze-drying, is a process employed to remove the water content from a substance by sublimation after it has been frozen, while preserving its structure and properties. The reagents can be lyophilized to a spheric lyo-bead or a compact lyo-cake format.
Lyophilization helps to extend the product’s shelf life, enhance the stability of the reagents at higher tempereatures, even when mixed together with primers and probes, and allows for convenient and accurate dispensing of the reagents. After lyophilization, the reagents are easily reconstituted with sample material.
Routine storage: -20 °C
On ice packs.
NB! Due to the use of ice packs, the shipping cost is approximately 200 euros (depending on package size and destination country).