10652_kit_104.jpg
NEW!

Some applications of this product  may require a license which is not provided by the purchase of this product.

For research use only. 

RT-LAMP Kit based on the Glycerol-Free SoliSD™ Bsm DNA Polymerase

In the new SoliSD™ Lyo-compatible RT-LAMP Kit all enzymes are compatible with freeze- and/or air-drying and contain the patented Stability TAG. The kit is provided in a flexible 5-vial format, with the unique 300X RT-Mix consisting of the blend of Solis BioDyne reverse transcriptases and RNase inhibitor.

  • Glycerol free format – compatible with lyophilization 
  • No NTC signal
  • Strong strand displacement activity of SoliSD™ Bsm
  • Perform RT-LAMP at 55-60°C with optimum at 59°C

NB! This is a special product. To place an order or ask for the price offer contact info@solisbiodyne.com.

Ordering

Choose Product Size
Catalog number

32-23-0000S

Quantity

€ 0.00

Log in to see Your Price

Details

Reagents Provided
Item Pcs. Vial size
SoliSD™ Glycerol-Free Bsm DNA Polymerase (40 U/μl) 1 2000 U | 50 μl
300x RT Mix 1 250 rxn | 25 µl
10x RT-LAMP Reaction Buffer 1 1 ml
100 mM MgSO4 1 0.5 ml
10x GC-rich Enhancer 1 0.5 ml
Overview

Description

SoliSD™ Glycerol-Free Bsm DNA Polymerase is based on the Large Fragment of the DNA Polymerase from the genus Bacillus smithii with functional similarity to other Bst and Bsm DNA polymerases. Implemented Stability TAG technology [1] ensures incredible stability for at least 1 month at 37°C. The enzyme has a strong strand displacement DNA polymerase activity and lacks 5’→3’ and 3’→5’ exonuclease activities.

SoliSD™ Lyo-compatible RT-LAMP Kit is provided in a 5-vial format, where composition gives flexibility for developing your own isothermal amplification assay.

[1] Kahre, O. et al., Compositions for increasing polypeptide stability and activity, and related methods, EP2501716B1 (2015) and US9321999B2 (2016).

Reagents:

  • SoliSD™ Glycerol-Free Bsm DNA Polymerase (40 U/μl)
  • 300x RT Mix (FIREScript®; SOLIScript®; RiboGrip®)
  • 10x RT-LAMP Reaction Buffer (200 mM Tris-HCl; 150 mM (NH4)2SO4; 1% Tween-20; 250 mM KCl; additives)
  • 100 mM MgSO4
  • 10x GC-rich Enhancer
Storage & Shipping
Storage

Routine storage: -20 °C 

Shipping

On ice packs.

NB! Due to the use of ice packs, the shipping cost is approximately 200 euros (depending on package size and destination country).