Some applications of this product  may require a license which is not provided by the purchase of this product.

For research use only. 

Convenient single vial format and sensitive 5-plex amplification for demanding one-step RT-qPCR experiments

  • 4x concentrated one-step RT-qPCR reagent for RNA detection
  • Convenient reaction setup with a single vial format
  • Amplify up to 5 targets within one reaction
  • UNG enzyme prevents carryover contamination and false positive results
  • Quick results thanks to fast extension rates of the in silico designed HOT SolisFAST® DNA Polymerase
  • Robust performance in inhibited conditions
  • RNase inhibitor included in the mix


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Reagents Provided
Item Pcs. Vial size
4x SOLIScript®️ Fast 1-step RT-qPCR Mix with UNG 1 50 rxn | 250 μl
Water, nuclease free 1 1.25 ml


SOLIScript® Fast 1-step RT-qPCR Mix with UNG is optimized for probe-based one-step RT-qPCR assays. It contains all components necessary in a single vial (except water, template, primers and probes) to perform cDNA synthesis and qPCR with up to 5 targets within around 1h of total reaction time. Inhibitor tolerance and compatibility with fast cycling enable flexible experiment design. Salini UNG® Uracil-N-Glycosylase is incorporated into the product along with dUTPs to prevent carryover contamination and false positive results.


  • Detection and quantification of RNA and DNA targets*
  • DNA/LNA hydrolysis probe-based assays
  • Suitable for singleplex and multiplex reactions (up to 5 plex)

* Read more from the application note.


Sample type: RNA and DNA
Product format: Reagent kit (nuclease-free water supplied in a separate tube)
Detection type: Probe-based
Reference dye: none
Compatible real-time instruments: The product is compatible with most qPCR cyclers. If using a ROX-dependent platform, switch off ROX normalization prior to the run. Check your cycler!*

*Version with ROX available upon request.


SOLIScript® Fast 1-step RT-qPCR Mix with UNG comprises:

  • 4x SOLIScript® Fast 1-step RT-qPCR Mix with UNG (SOLIScript® Reverse Transcriptase, RiboGrip® RNase Inhibitor, HOT SolisFAST® DNA polymerase, Salini UNG® Uracil-N-Glycosylase, a unique reaction buffer with dNTPs (including dUTPs), 22.0 mM MgCl2 (1x RT-qPCR solution – 5.5 mM MgCl2) and additives to maximize PCR efficiency).
  • Water, nuclease-free
Reliable results over a wide dynamic range
Increase throughput with 5-plex amplification
Strong amplification of both RNA and DNA targets
Prevent carryover contamination and false positive results with UNG
Tolerance to common PCR inhibitors: robust performance in inhibited conditions
Competitive performance
Storage & Shipping

Routine storage: -20 °C 

Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of SOLIScript® Fast 1-step RT-qPCR Mix with UNG.


At room temperature.