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Some applications of this product  may require a license which is not provided by the purchase of this product.

For research use only. 

Novel in-silico engeneered, protein-based RNase inhibitor

  • included in our cDNA synthesis kits and 1-step RT-qPCR kits
  • reaction set-up and shipment without ice

Ordering

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Catalog number

06-22-01000

Quantity

€ 75.00

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Details

Overview

Description

RiboGrip™ RNase Inhibitor is an in silico-designed protein-based ribonuclease inhibitor, which inactivates RNase A.

RiboGrip™ inhibits the activity of ribonuclease A by noncovalently binding in a non-competitive mode at a ratio of 1:1. This novel inhibitor  has enhanced stability at room temperature with no loss of activity for up to 1 month.

Applications

Preventing RNA degradation in the following applications:

  • First-strand cDNA synthesis
  • RT-PCR
  • RT-qPCR
  • in vitro transcription and translation

Properties

Concentration: 40 U/µl

Source: Purified from an E.coli strain that carries an overproducing plasmid containing a RiboGrip™ RNase Inhibitor gene.

Storage and dilution buffer: 50% glycerol (v/v), 20 mM Tris-HCl pH 7.5, 100 mM KCl, 0.1 mM EDTA, 5 mM DTT and stabilizers.

Recommendations for use: RiboGrip RNase Inhibitor™ is used generally at a final reaction concentration of 1 U/μl. Inactivated by heating at 85°C for 5 min.

Included in FIREScript® and SOLIScript® cDNA Synthesis Kits and Mixes, and in all SOLIScript® 1-step RT-PCR kits

Related Resources
Storage & Shipping
Storage

Routine storage: -20ºC. 

Shipping

Shipping and temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of RiboGrip RNase Inhibitor.