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RiboGrip®️ Glycerol-Free RNase Inhibitor (220 U/µl)

10619_06-29_RiboGrip-GF-product-photo_1.jpg

Some applications of this product  may require a license which is not provided by the purchase of this product.

For research use only. 

Novel in silico engineered, protein-based glycerol-free RNase inhibitor

  • Glycerol free format – compatible with lyophilization and air-drying
  • Exceptional stability – stable for up to 60 °C
  • Unique high concentration formulation
  • Efficient protection of RNA at low DTT concentrations
  • Clients report excellent performance following lyophilization
  • Find out more from the video HERE. 

Ordering

Available sizes
Catalog number

06-29-0000S

Quantity

€ 0.00

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Details

Overview

Description

RiboGrip® Glycerol-Free RNase Inhibitor (220 U/µl) is an in silico-designed protein-based ribonuclease inhibitor, which inactivates RNase A, RNase B and RNase C.

RiboGrip® inhibits the activity of ribonuclease A by noncovalently binding in a non-competitive mode at a ratio of 1:1. It is primarily used to prevent RNA degradation by contaminating RNases in various assays that use RNA sample materials, such as first-strand cDNA synthesis, RT-(q)PCR, RT-LAMP, etc.

RiboGrip® also includes a genetic modification - Stability TAG - Solis BioDyne’s proprietary and patented polypeptide stabilization technology [1]. Stability TAG makes RiboGrip® extremely tolerant to higher temperatures and enables room temperature shipping as well as effective use in assays requiring high incubation temperatures.

Highly concentrated 220 U/µl and glycerol-free formulation ensures compatibility with lyophilization and air-drying. This product does not contain excipients, please add your own as required or contact us for more information.

Our RiboGrip® is also compatible with Illumina's protocols (particularly single-cell sequencing). See HERE.

[1] Kahre, O. et al., Compositions for increasing polypeptide stability and activity, and related methods, EP2501716B1 (2015) and US9321999B2 (2016)

Applications

Compatible with lyophilisation and air-drying. Preventing RNA degradation in a variety of applications including:

  • Assays involving lyophilisation or air-drying.
  • First-strand cDNA synthesis
  • RT-PCR, RT-qPCR, RT-LAMP
  • In vitro transcription and translation
  • RNA isolation and purification
  • RNA sequencing

Properties

Concentration: 220 U/µl

Origin: Recombinant mammalian chimeric protein

Source: Purified from an E. coli strain that carries an overproducing plasmid containing a RiboGrip® RNase Inhibitor gene.

Molecular weight: 47 kD monomer

Unit definition: One unit is defined as the amount of protein required to inhibit the activity of 5 ng of RNAse A by 50%. Activity is measured by the inhibition of hydrolysis of cytidine 2',3'‐cyclic monophosphate by RNase A.

Storage buffer: 25 mM Tris-HCl pH 7.5, 100 mM KCl, 0.1 mM EDTA, 5 mM DTT and stabilizers.

Specificity: RNase A, RNase B, RNase C 

Recommendations for use: 0,5-2.5 U/μl final reaction concentration

Related Resources
Storage & Shipping
Storage

Routine storage: -20ºC.

Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of the reagents.

Shipping

At room temperature.