Some applications of this product may require a license which is not provided by the purchase of this product.
For research use only.
HOT SolisAcura™ Exo(-) DNA Polymerase has been genetically modified to be extremely sensitive to mismatches on the 3’ ends of primers, resulting in superior performance in SNP detection and allele discrimination. It has 10-15x higher fidelity compared to wild-type Taq polymerase, which comes from choosing nucleotides more accurately. Additionally, there is no 3′→5′ exonuclease (proofreading) activity with this enzyme. What is more, the Exo(-) in the enzyme’s name refers to the polymerase not possessing 5′→3′ exonuclease activity.
The novel polymerase is also engineered to have faster synthesis rates and is inherently inhibitor tolerant, ensuring excellent performance with crude or challenging samples. It comes with chemical hot-start that improves the specificity and accuracy of the reaction, minimizes mispriming and extension from non-specifically annealed primers and primer-dimers.
Additionally, the HOT SolisAcura™ Exo(-) DNA Polymerase is room temperature stable for up to 1 month due to the use of Stability TAG – Solis BioDyne’s proprietary and patented polypeptide stabilization technology that is incorporated into the enzyme, enabling environmentally friendly ambient temperature shipping [1]. Room temperature stability also enables maximum convenience allowing reactions to be set up without ice at room temperature.
[1] Kahre, O. et al., Compositions for increasing polypeptide stability and activity, and related methods, EP2501716B1 (2015) and US9321999B2 (2016)
Sample type: DNA
Product format: Kit
Hot start: Yes, chemical (15-minute activation)
Fidelity: 10-15x compared to Taq polymerase
Detection type: end-point analysis
Targets: 1-18+
Routine storage: -20 ºC.
Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of the reagents.
At room temperature.
Probe genotyping mastermix based on a novel SolisAcura™ Exo(+) DNA polymerase with increased limit of detection for allele discrimination.
This master mix has been developed for TaqMan® hydrolysis probes but is also suitable for other hydrolysis probe types.
Accurate, inhibitor tolerant and high-specificity cassette-based genotyping mix for precise bi-allelic SNP detection.
