Some applications of this product may require a license which is not provided by the purchase of this product.
For research use only.
FIREScript® ULTRA H(+) Reverse Transcriptase (RT) is an in silico engineered RNA-directed DNA polymerase that can synthesize a complementary DNA strand from ssRNA or ssDNA. It is designed for fast, efficient, and reproducible cDNA synthesis.
The enzyme is active across a broad temperature range (37°C–65°C) and contains a functional RNase H domain, enabling efficient RNA template processing and supporting high-performance RT-qPCR workflows. Its rapid kinetics and optimized turnover contribute to consistently low Cq values, improving detection sensitivity and enabling earlier target identification.
FIREScript® ULTRA H(+) demonstrates excellent stability at room temperature and at 37°C, retaining activity for up to 1 month due to the use of Stability TAG – Solis BioDyne’s proprietary and patented polypeptide stabilization technology that is incorporated into the enzyme, enabling environmentally friendly ambient temperature shipping [1]. In addition, the enzyme supports benchtop workflow robustness, remaining stable at room temperature for up to 24 hours during reaction setup, enabled by its inherent warm-start properties. Room temperature stability also enables maximum convenience allowing reactions to be set up without ice at room temperature.
The combination of great reaction speed and superior sensitivity makes FIREScript® ULTRA H(+) RT well suited for routine, high-throughput, and RNA detection workflows in diagnostics.
For optimal performance, the use of RiboGrip® RNase Inhibitor is strongly recommended.
[1] Kahre, O. et al., Compositions for increasing polypeptide stability and activity, and related methods, EP2501716B1 (2015) and US9321999B2 (2016)
Sample type: RNA
Final product: cDNA (first strand)
Product format: Enzyme with all components included reagent kit
Enzyme: FIREScript® ULTRA H(+) Reverse Transcriptase
Routine storage: -20 ºC.
Temporary storage for up to 1 month at room temperature has no detrimental effects on the quality of the reagents.
Shipping at room temperature.
Highly specific and reproducible dye-based qPCR Mix with blue visualization dye for easy pipetting. Suitable for ROX-dependent and ROX-independent qPCR cyclers.
FIREScript® FLEX H(-) Reverse Transcriptase (RT) is an in silico engineered RNA-directed DNA polymerase designed for high-sensitivity cDNA synthesis and robust performance under challenging conditions.
FIREScript® ULTRA H(+) Reverse Transcriptase (RT) is an in silico engineered RNA-directed DNA polymerase designed for fast, efficient, and reproducible cDNA synthesis.
All-in-one kit for full-length cDNA synthesis from complex, structured RNA with new gen FIREScript® FLEX H(-) Reverse Transcriptase.
RiboGrip® RNase Inhibitor (220 U/µl) is an in silico-designed protein-based ribonuclease inhibitor with enhanced stability. Effective protection against RNase A, RNase B and RNase C.
