Some applications of this product may require a license which is not provided by the purchase of this product.
For research use only.
Extremely fast end-point PCR mix for robust and accurate target detection for single- and multiplex reactions
SolisFAST® Master Mix is a 5x-concentrated, ready-to-use solution for fast singleplex and multiplex endpoint PCR assays. The mix allows amplification of up to 5 kb fragments from low complexity DNA templates (e.g. lambda, plasmid DNA), and up to 3 kb from complex DNA templates (e.g. human genomic DNA).
Combining our novel in-silico designed SolisFAST® DNA Polymerase and optimized buffer, this mix offers robust PCR, accurate target detection, ice-free shipping and reaction set-up.
The mix can be loaded directly to gel for post PCR analysis.
Our SolisFAST® Master Mix works the fastest with Eppendorf Mastercycler X50s and Eppendorf Fast PCR Tube Strips. Find out more HERE.*
* The cooperation of Solis BioDyne and Eppendorf is limited to SolisFAST® product range and Eppendorf Mastercycler X50 in selected regions: China, Asia Pacific, Africa, Middle East.
Concentration: 5x
Hot-start: yes, 2 minutes initial incubation at 98 °C
Ready-to-load: yes, includes two loading dyes
Cloning Type: T/A cloning
Amplicon Size: up to 5 kb
Targets: 1-18 targets, depending on target length
SolisFAST® DNA Polymerase: In silico designed analogue of Taq DNA polymerase with enhanced stability at room temperature, fast hot-start and faster extension rates compared to the wild-type Taq DNA polymerase.
PCR buffer: Includes 17.5 mM MgCl2 (1x PCR solution 3.5 mM MgCl2), dNTPs (dATP, dCTP, dGTP, dTTP), additives that maximize efficiency of PCR
Green loading dye1: Mix of blue and yellow dye to allow tracking of DNA during electrophoresis.
1The mix is not recommended for use in applications where spectrophotometric measurements are necessary (e.g. Sanger sequencing). Migration speed: blue dye – TBE: ~3.5 kb, TAE: ~4.5 kb; yellow dye – TBE: ~35 bp, TAE: ~45 bp).
FIREPol® and HOT FIREPol® DNA Polymerases and Master Mixes, as well as SolisFAST® Master Mix are compatible with most restriction enzymes without cleaning up the PCR reaction product.
SolisFAST® Master Mix with UNG contains dUTP instead of dTTP leading to the generation of PCR products containing uracil. Check your restriction enzyme for the ability to digest U-containing DNA amplicons.
Products generated by our FIREPol®, HOT FIREPol® DNA Polymerases and Master Mixes, as well as SolisFast® Master Mixes may be used for Sanger sequencing.
Prior to the sequencing, the products should be purified to remove excess primers and nucleotides (enzymatically with ExoI/SAP or Spin column-based nucleic acid purification).
Products generated with 5x HOT FIREPol® Blend Master Mix should be cleaned up utilising column-based nucleic acid purification method, because this mix contains proofreading enzyme with 3’-5’ exonuclease activity and may degrade your sequencing primer.
Please note that Ready to Load versions of our Master Mixes are not recommended for use in applications where spectro-photometric measurements (absorbance or fluorescence) are necessary because tracking dyes can interfere with these applications.
Solis BioDyne products should be stored at -20°C.
Shipping and temporary storage for up to 1 month at room temperature (*15−25°C) has no detrimental effects on the quality of Solis BioDyne reagents.
Freeze-thaw stability is tested for each product. Most PCR and qPCR products have passed 30 freeze-thaw cycles with no changes in performance. Specific information is found in Storage and Shipping conditions of each product.
When stored and handled under the recommended conditions, full activity of the reagents is retained until the Expiry Date printed on the tube label.
*World Health Organization (2003). Guidelines for the Storage of Essential Medicines and Other Health Commodities.
Routine storage at -20 °C until expiry date
The mix can be stored at +4 °C for up to 1 year
Stability at room temperature (15 − 25 °C) for 2 months
Freeze-thaw stability: 30 cycles
At room temperature
