Some applications of this product may require a license which is not provided by the purchase of this product.
For research use only.
Convenient kit format for performing highly specific cDNA synthesis and probe-based qPCR in a single tube. The kit contains qPCR mix, SOLIScript® RT enzyme mix with RiboGrip® RNase inhibitor, and nuclease-free water.
SOLIScript® 1-step Multiplex Probe Kit is optimized for probe based one-step RT real-time quantitative PCR assays. It contains all the components necessary (except template, primers, and probes) to perform cDNA synthesis and probe based qPCR in a single tube.
Reagent composition is optimized for excellent performance in highly multiplexed reactions while making sure you don't lose in sensitivity compared to singleplex or duplex assays.
Sample type: RNA
Product format: Reagent kit
Detection type: Probe-based
Reference dye: none
Compatible real-time instruments: The kit is compatible with qPCR cyclers that do not require an internal reference dye (e.g. ROX) for normalization of fluorescent signal. Check Your cycler!
5x One-step Multiplex Probe Mix with HOT FIREPol® DNA polymerase, reaction buffer, dNTPs including dUTP, 15 mM MgCl2 (1x qPCR solution - 3 mM MgCl2)
40x One-step SOLIScript® Mix with SOLIScript® Reverse Transcriptase, RiboGrip® RNase Inhibitor
Water, nuclease free
RiboGrip™ is not directly associated with tolerance to common inhibitors, however, it helps to keep RNA intact and prevent RNA degradation because RiboGrip is inactivating ribonuclease enzymes
ROX (carboxy-X-rhodamine) is one of the passive reference dyes providing a constant fluorescent signal that is used for signal normalization during the amplification cycles. The emission recorded from a reference dye during the baseline cycles is used to normalize the emission recorded from the reporter (e.g. EvaGreen®, SolisGreen®, SYBR® Green, etc.) in later cycles in ROX-dependent real-time PCR systems (e.g. Applied Biosystems 5700, 7000, 7300, 7700, 7900HT, StepOne™, StepOnePlus™). Reference dye compensates for small fluorescent fluctuations and well-to-well variations that may occur.
In Solis BioDyne qPCR mixes we use technology, based on ROX, that allows us to use same mix for high- and low- ROX requiering cyclers.
Please check cycler-mix compatibility here if not sure.
Solis BioDyne products should be stored at -20°C.
Shipping and temporary storage for up to 1 month at room temperature (*15−25°C) has no detrimental effects on the quality of Solis BioDyne reagents.
Freeze-thaw stability is tested for each product. Most PCR and qPCR products have passed 30 freeze-thaw cycles with no changes in performance. Specific information is found in Storage and Shipping conditions of each product.
When stored and handled under the recommended conditions, full activity of the reagents is retained until the Expiry Date printed on the tube label.
*World Health Organization (2003). Guidelines for the Storage of Essential Medicines and Other Health Commodities.
Routine storage: -20 ºCTemporary storage for up to 1 month at room temperature has no detrimental effects on the quality of the reagents.
At room temperature
Probe-based qPCR Mix optimized for amplifying multiple targets in a single reaction in real-time quantitative PCR assays
Sensitive and highly specific cDNA synthesis and qPCR in a single tube. Suitable for ROX and no ROX cyclers.
A flexible and robust cDNA synthesis kit contains all the reagents you need for first-strand cDNA synthesis. Individual components enable thorough assay optimization.
All-inclusive and the most convenient product format for first-strand cDNA synthesis using gene-specific primers. A combined enzyme mix (SOLIScript® and RiboGrip®) and a reaction premix in separate tubes. Primers not included.
