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Sensitive and highly specific 1-step RT-qPCR reagents optimized for SARS-CoV-2 detection are perfect for variant discrimination.

Convenient kit format for performing highly specific cDNA synthesis and probe-based qPCR in a single tube.

SOLIScript® 1-step CoV Kit

The kit contains qPCR mix, SOLIScript® RT enzyme mix with RiboGrip™ RNase inhibitor, and nuclease-free water. Just combine with your selected primers and probes.

  • Sensitivity down to 2 copies per reaction
  • Room temperature stability for 30 days in liquid format
  • Superior specificity
  • Multiplexing up to 4 targets in 1 reaction
  • RNase inhibitor included in the kit
  • Wide compatibility covering most qPCR cyclers

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Product Details

Reagents Provided
Item Pcs. Vial size
40x One-step SOLIScript® CoV Mix 1 250 rxn | 125 µl
5x One-step Probe CoV Mix 1 250 rxn | 1 ml
Water, nuclease free 1 5 ml
Overview

Description

SOLIScript® 1-step CoV Kit is optimized for highly sensitive detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) viral RNA by one-step real-time RT-PCR (RT-qPCR).

The Kit is suitable for the detection of up to four targets simultaneously and can be used with all common qPCR cyclers. Follow recommendations specific for a selected SARS-CoV-2 diagnostic panel, and instrument-specific guidelines. Contact us if a passive reference dye is required for signal normalization.

SARS-CoV-2 primers and probes, applicable positive and internal extraction controls are not provided with the Kit and should be supplied by the user.

SOLIScript® 1-step CoV Kit is tested and validated for SARS-CoV-2 detection by diagnostic laboratories. Please see our recommendations for US-CDC and Berlin-Charité protocols. 

Note! Amplification of more than one target in a single reaction may require considerable optimization, individual assays must be validated prior to multiplexing. For multiplex reactions, design primer pairs with similar annealing temperatures for all targets to be amplified. Self-complementarity of primers should be avoided.

Applications

Detection and quantification of RNA targets with qPCR (including SARS-CoV-2)

DNA/LNA hydrolysis probe-based assays

Suitable for singleplex and multiplex reactions

Properties

Sample type: RNA

Product format: Reagent kit 

Detection type: Probe-based

Reference dye: not included 

Compatible real-time instruments: Most qPCR platforms. Check Your cycler!

Reagents 

5x One-step Probe CoV Mix  with HOT FIREPol® DNA polymerase, reaction buffer, dNTPs, 15 mM MgCl2 (1x qPCR solution - 3 mM MgCl2)

40x One-step SOLIScript® CoV Mix with SOLIScript® Reverse Transcriptase and RiboGrip™ RNase Inhibitor 

Water, nuclease free

Performance validated with clinical samples
Highly sensitive detection, evaluated with SARS-CoV-2 synthetic single stranded RNA
Related Resources

FAQ

Does RiboGrip help with inhibitor resistance?

RiboGrip™ is not directly associated with tolerance to common inhibitors, however, it helps to keep RNA intact because RiboGrip inhibits Rnase H.

Which primes, probes are recommended for COVID-19 detection with Your 1-step kit?

The Kit has been tested and validated by diagnostic laboratories using primer-probe panels recommended by Charité, (Germany) and CDC (USA) protocols according to WHO “Coronavirus disease (COVID-19) technical guidance: Laboratory testing for 2019-nCoV in humans”, section: “Molecular assays to diagnose COVID-19”; subsection: “In-house developed molecular assays”

Are primers, probes and positive control included in the 1-step RT-qPCR CoV kit?

SARS-CoV-2 primers and probes, applicable positive and internal extraction controls are not provided with the Kit and should be supplied by the user.

Why do certain kits contain a ROX?

ROX (carboxy-X-rhodamine) is one of the passive reference dyes providing a constant fluorescent signal that is used for signal normalization during the amplification cycles. The emission recorded from a reference dye during the baseline cycles is used to normalize the emission recorded from the reporter (e.g. EvaGreen®, SolisGreen®, SYBR® Green, etc.) in later cycles in ROX-dependent real-time PCR systems (e.g. Applied Biosystems 5700, 7000, 7300, 7700, 7900HT, StepOne™, StepOnePlus™). Reference dye compensates for small fluorescent fluctuations and well-to-well variations that may occur.

In Solis BioDyne qPCR mixes we use technology, based on ROX, that allows us to use same mix for high- and low- ROX requiering cyclers. 

Please check cycler-mix compatibility here if not sure.

Storage & Shipping
Storage

Routine storage: -20oC

Temporary storage for up to 30 days at room temperature has no detrimental effects on the quality of SOLIScript® 1-step CoV Kit

Shipping

At room temperature

Custom Solutions?

Need something custom and out-of-the-box? Your needs are our priority. To ensure quick development and go-to-market time you can leverage our dedicated support and development throughout the process. Please contact us if you have a specific requirement but cannot find the best solution among our catalog products. We may be able to offer you bulk products, tailored product sizes, and formats, or specific formulations. We are flexible and dedicated to meeting your needs!

Quality has always been the core value of our work. To ensure we match the high-quality requirements of our partners in the research and diagnostic sector, we implemented the ISO 13485 standard in 2018 to complement the ISO 9001 certificate we acquired in 2007.

Testimonials

Strategic cooperation with the Solis BioDyne team has been one of the most efficient we have experienced during our COVID-19 diagnostic kits products development. Their room temperature stable master mix is an excellent product that has helped us to reach higher sensitivity and stability of our qPCR diagnostic kits. 

PETER KILIAN
Chief Operating Officer at MultiplexDX

We chose SOLIScript 1-step CoV kit for SARS-CoV-2 wastewater screening project because we anticipated difficulties in the global supply chain and we already had experience with Solis BioDyne products. In addition, we believe in using local suppliers when possible and reducing our ecological footprint.

VELJO KISAND, PhD
Associate Professor of Molecular Ecology, University of Tartu

Want to try it out?

Test the sample, consult with us if You need help in assay optimisation and You are set for success!

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